dc.creator | Olmedo Verd, Elvira de | es |
dc.creator | Valladares Ruiz, Ana | es |
dc.creator | Flores García, Enrique | es |
dc.creator | Herrero Moreno, Antonia | es |
dc.creator | Muro Pastor, Alicia María | es |
dc.date.accessioned | 2017-07-24T08:59:12Z | |
dc.date.available | 2017-07-24T08:59:12Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | Olmedo Verd, E., Valladares Ruiz, A., Flores García, E., Herrero, A. y Muro Pastor, A.M. (2008). Role of two NtcA-binding sites in the complex ntcA gene promoter of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. Journal of Bacteriology, 190 (22), 7584-7590. | |
dc.identifier.issn | 0021-9193 (impreso) | es |
dc.identifier.issn | 1098-5530 (electronico) | es |
dc.identifier.uri | http://hdl.handle.net/11441/62948 | |
dc.description.abstract | Anabaena sp. strain PCC 7120 is a filamentous cyanobacterium that fixes N2 in specialized cells called heterocysts, which differentiate from vegetative cells in a process that requires the nitrogen control transcription factor NtcA. 2-Oxoglutarate-stimulated binding of purified NtcA to wild-type and modified versions of the ntcA gene promoter from Anabaena sp. was analyzed by mobility shift and DNase I footprinting assays, and the role of NtcA-binding sites in the expression of the ntcA gene during heterocyst differentiation was studied in vivo by using an ntcA-gfp translational fusion and primer extension analysis. Mutation of neither of the two identified NtcA-binding sites eliminated localized expression of ntcA in proheterocysts, but mutation of both sites led to very low, nonlocalized expression. | es |
dc.description.sponsorship | Ministerio de Educación y Ciencia BFU2004-00872 BFU2007-60457 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Journal of Bacteriology, 190 (22), 7584-7590. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Role of two NtcA-binding sites in the complex ntcA gene promoter of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120 | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | BFU2004-00872 | es |
dc.relation.projectID | BFU2007-60457 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1128/JB.00856-08 | es |
dc.identifier.doi | 10.1128/JB.00856-08 | es |
idus.format.extent | 7 p. | es |
dc.journaltitle | Journal of Bacteriology | es |
dc.publication.volumen | 190 | es |
dc.publication.issue | 22 | es |
dc.publication.initialPage | 7584 | es |
dc.publication.endPage | 7590 | es |
dc.contributor.funder | Ministerio de Educación y Ciencia (MEC). España | |