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dc.creatorTorres Torrelo, Julioes
dc.creatorTorres Ruiz, Blases
dc.creatorCarrascal Moreno, María Liviaes
dc.date.accessioned2017-04-10T12:33:57Z
dc.date.available2017-04-10T12:33:57Z
dc.date.issued2014
dc.identifier.issn0022-3751es
dc.identifier.urihttp://hdl.handle.net/11441/57430
dc.description.abstractThe neuronal input–output function depends on recruitment threshold and gain of the firing frequency–current (f–I) relationship. These two parameters are positively correlated in ocular motoneurons (MNs) recorded in alert preparation and inhibitory inputs could contribute to this correlation. Phasic inhibition mediated by γ-amino butyric acid (GABA) occurs when a high concentration of GABA at the synaptic cleft activates postsynaptic GABAA receptors, allowing neuronal information transfer. In some neuronal populations, low concentrations of GABA activate non-synaptic GABAA receptors and generate a tonic inhibition, which modulates cell excitability. This study determined how ambient GABA concentrations modulate the input–output relationship of rat oculomotor nucleus MNs. Superfusion of brain slices with GABA (100 μm) produced a GABAA receptor-mediated current that reduced the input resistance, increased the recruitment threshold and shifted the f–I relationship rightward without any change in gain. These modifications did not depend on MN size. In absence of exogenous GABA, gabazine (20 μm; antagonist of GABAA receptors) abolished spontaneous inhibitory postsynaptic currents and revealed a tonic current in MNs. Gabazine increased input resistance and decreased recruitment threshold mainly in larger MNs. The f–I relationship shifted to the left, without any change in gain. Gabazine effects were chiefly due to MN tonic inhibition because tonic current amplitude was five-fold greater than phasic. This study demonstrates a tonic inhibition in ocular MNs that modulates cell excitability depending on cell size. We suggest that GABAA tonic inhibition acting concurrently with glutamate receptors activation could reproduce the positive covariation between threshold and gain reported in alert preparation.es
dc.description.sponsorshipEspaña, Ministerio de Ciencia y Tecnología BFU 2009-07867es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherPhysiological Societyes
dc.relation.ispartofJournal of Physiology, 592 (Pt 22), 5047-5064.es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleModulation of the input–output function by GABAA receptor-mediated currents in rat oculomotor nucleus motoneuronses
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Fisiologíaes
dc.relation.projectIDBFU 2009-07867es
dc.relation.publisherversionhttp://dx.doi.org/10.1113/jphysiol.2014.276576es
dc.identifier.doi10.1113/jphysiol.2014.276576es
idus.format.extent18 p.es
dc.journaltitleJournal of Physiologyes
dc.publication.volumen592es
dc.publication.issuePt 22es
dc.publication.initialPage5047es
dc.publication.endPage5064es
dc.contributor.funderMinisterio de Ciencia y Tecnología (MCYT). España

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Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional