dc.creator | García Rubio, María Luisa | es |
dc.creator | Pérez Calero, Carmen | es |
dc.creator | Barroso Ceballos, Sonia Inés | es |
dc.creator | Tumini, Emanuela | es |
dc.creator | Herrera Moyano, Emilia | es |
dc.creator | Aguilera López, Andrés | es |
dc.date.accessioned | 2017-03-14T12:53:53Z | |
dc.date.available | 2017-03-14T12:53:53Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | García Rubio, M.L., Pérez Calero, C., Barroso Ceballos, S.I., Tumini, E., Herrera Moyano, E. y Aguilera López, A. (2015). The Fanconi Anemia Pathway Protects Genome Integrity from R-loops. Plos Genetics, 11 (11), e1005674. | |
dc.identifier.issn | 1553-7390 | es |
dc.identifier.uri | http://hdl.handle.net/11441/55830 | |
dc.description.abstract | Co-transcriptional RNA-DNA hybrids (R loops) cause genome instability. To prevent harmful R loop accumulation, cells have evolved specific eukaryotic factors, one being the BRCA2 double-strand break repair protein. As BRCA2 also protects stalled replication forks and is the FANCD1 member of the Fanconi Anemia (FA) pathway, we investigated the FA role in R loop-dependent genome instability. Using human and murine cells defective in FANCD2 or FANCA and primary bone marrow cells from FANCD2 deficient mice, we show that the FA pathway removes R loops, and that many DNA breaks accumulated in FA cells are R loop-dependent. Importantly, FANCD2 foci in untreated and MMC-treated cells are largely R loop dependent, suggesting that the FA functions at R loop-containing sites. We conclude that co-transcriptional R loops and R loop-mediated DNA damage greatly contribute to genome instability and that one major function of the FA pathway is to protect cells from R loops. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Public Library of Science | es |
dc.relation.ispartof | Plos Genetics, 11 (11), e1005674. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | The Fanconi Anemia Pathway Protects Genome Integrity from R-loops | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | http://dx.doi.org/ 10.1371/journal.pgen.1005674 | es |
dc.identifier.doi | 10.1371/journal.pgen.1005674 | es |
idus.format.extent | 18 p. | es |
dc.journaltitle | Plos Genetics | es |
dc.publication.volumen | 11 | es |
dc.publication.issue | 11 | es |
dc.publication.initialPage | e1005674 | es |