Artículo
NRT2.4 and NRT2.5 Are Two Half-Size Transporters from the Chlamydomonas NRT2 Family
Autor/es | Higuera, Jose Javier
Calatrava, Victoria González Sánchez, Zaira Mariscal, Vicente Siverio Exposito, Jose Manuel Fernández, Emilio Galván, Aurora |
Departamento | Universidad de Sevilla. Instituto de Bioquímica Vegetal y Fotosíntesis IBVF – CIC Cartuja |
Fecha de publicación | 2016 |
Fecha de depósito | 2016-11-10 |
Publicado en |
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Resumen | The NRT2 transporters mediate High Affinity Nitrate/NitriteTransport (HAN/NiT), which are essential for nitrogen acquisition from these inorganic forms. The NRT2 proteins are encoded by a multigene family in plants, and ... The NRT2 transporters mediate High Affinity Nitrate/NitriteTransport (HAN/NiT), which are essential for nitrogen acquisition from these inorganic forms. The NRT2 proteins are encoded by a multigene family in plants, and contain 12 transmembrane-spanning domains. Chlamydomonas reinhardtii has six NRT2, two of which (NRT2.5 and NRT2.4) are located in Chromosome III, in tandem head to tail. cDNAs for these genes were isolated and their sequence revealed that they correspond to half-size NRT2 transporters each containing six transmembrane domains. NRT2.5 has long N- and C- termini sequences without known homology. NRT2.4 also contains long termini sequences but smaller than NRT2.5. Expression of both studied genes occurred at a very low level, slightly in darkness, and was not modified by the N or C source. Silencing of NRT2.4 by specific artificial miRNA resulted in the inhibition of nitrite transport in the absence of other HANNiT (NRT2.1/NAR2) in the cell genetic background. Nitrite transport activity in the Hansenula polymorpha Dynt::URA3 Leu2 mutant was restored by expressing CrNRT2.4. These results indicate that half-size NRT2 transporters are present in photosynthetic organisms and that NRT2.4 is a HANiT. |
Cita | Higuera, J.J., Calatrava, V., González, Z., Mariscal, V., Siverio Exposito, J.M., Fernández, E. y Galván, A. (2016). NRT2.4 and NRT2.5 Are Two Half-Size Transporters from the Chlamydomonas NRT2 Family. Agronomy, 6 (1) |
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