dc.creator | Kressler, Dieter | es |
dc.creator | Cruz Díaz, Jesús de la | es |
dc.creator | Rojo, Manuel | es |
dc.creator | Linder, Patrick | es |
dc.date.accessioned | 2016-08-08T11:04:11Z | |
dc.date.available | 2016-08-08T11:04:11Z | |
dc.date.issued | 1998 | |
dc.identifier.citation | Kressler, D., Cruz Díaz, J.d.l., Rojo, M. y Linder, P. (1998). Dbp6p is an essential putative ATP-dependent RNA helicase required for 60S-ribosomal-subunit assembly in Saccharomyces cerevisiae. Molecular and Cellular Biology, 18 (4), 1855-1865. | |
dc.identifier.issn | 0270-7306 | es |
dc.identifier.uri | http://hdl.handle.net/11441/44285 | |
dc.description.abstract | A previously uncharacterizedSaccharomyces cerevisiaeopen reading frame, YNR038W, was analyzed in the
context of the European Functional Analysis Network. YNR038W encodes a putative ATP-dependent RNA helicase of the DEAD-box protein family and was therefore namedDBP6(DEAD-box protein 6). Dbp6p is essential
for cell viability. In vivo depletion of Dbp6p results in a deficit in 60S ribosomal subunits and the appearance
of half-mer polysomes. Pulse-chase labeling of pre-rRNA and steady-state analysis of pre-rRNA and mature
rRNA by Northern hybridization and primer extension show that Dbp6p depletion leads to decreased production of the 27S and 7S precursors, resulting in a depletion of the mature 25S and 5.8S rRNAs. Furthermore,
hemagglutinin epitope-tagged Dbp6p is detected exclusively within the nucleolus. We propose that Dbp6p is
required for the proper assembly of preribosomal particles during the biogenesis of 60S ribosomal subunits,
probably by acting as an rRNA helicase | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Molecular and Cellular Biology, 18 (4), 1855-1865. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Dbp6p is an essential putative ATP-dependent RNA helicase required for 60S-ribosomal-subunit assembly in Saccharomyces cerevisiae | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | http://mcb.asm.org/content/18/4/1855.full.pdf+html | es |
idus.format.extent | 11 p. | es |
dc.journaltitle | Molecular and Cellular Biology | es |
dc.publication.volumen | 18 | es |
dc.publication.issue | 4 | es |
dc.publication.initialPage | 1855 | es |
dc.publication.endPage | 1865 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/44285 | |