dc.creator | Gaillard, Hélène | |
dc.creator | Aguilera López, Andrés | |
dc.date.accessioned | 2015-10-01T10:52:02Z | |
dc.date.available | 2015-10-01T10:52:02Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Gaillard, H. y Aguilera López, A. (2014). Cleavage factor I links transcription termination to DNA damage response and genome integrity maintenance in Saccharomyces cerevisiae. PLoS Genetics, 10 (3), 1-12. | es |
dc.identifier.issn | 1553-7404 | es |
dc.identifier.issn | 1553-7390 | es |
dc.identifier.uri | http://hdl.handle.net/11441/29104 | |
dc.description.abstract | During transcription, the nascent pre-mRNA undergoes a series of processing steps before being exported to the cytoplasm. The 3′-end processing machinery involves different proteins, this function being crucial to cell growth and viability in eukaryotes. Here, we found that the rna14-1, rna15-1, and hrp1-5 alleles of the cleavage factor I (CFI) cause sensitivity to UV-light in the absence of global genome repair in Saccharomyces cerevisiae. Unexpectedly, CFI mutants were proficient in UV-lesion repair in a transcribed gene. DNA damage checkpoint activation and RNA polymerase II (RNAPII) degradation in response to UV were delayed in CFI-deficient cells, indicating that CFI participates in the DNA damage response (DDR). This is further sustained by the synthetic growth defects observed between rna14-1 and mutants of different repair pathways. Additionally, we found that rna14-1 suffers severe replication progression defects and that a functional G1/S checkpoint becomes essential in avoiding genetic instability in those cells. Thus, CFI function is required to maintain genome integrity and to prevent replication hindrance. These findings reveal a new function for CFI in the DDR and underscore the importance of coordinating transcription termination with replication in the maintenance of genomic stability. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Public Library of Science | es |
dc.relation.ispartof | PLoS Genetics, 10(3), 1-12 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | DNA damage | es |
dc.subject | Transcriptional termination | es |
dc.subject | DNA replication | es |
dc.subject | DNA transcription | es |
dc.subject | DNA repair | es |
dc.subject | Damage mechanics | es |
dc.subject | Genetics networks | es |
dc.subject | Protein structure networks | es |
dc.title | Cleavage factor I links transcription termination to DNA damage response and genome integrity maintenance in Saccharomyces cerevisiae | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | http://dx.doi.org/10.1371/journal.pgen.1004203 | es |
dc.identifier.doi | http://dx.doi.org/10.1371/journal.pgen.1004203 | es |
dc.journaltitle | PLoS Genetics | es |
dc.publication.volumen | 10 | es |
dc.publication.issue | 3 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 12 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/29104 | |