Application of flow focusing to the break-up of a magnetite suspension jet for the production of paramagnetic microparticles
|Author/s||Martín Banderas, Lucía
González Prieto, Román
Rodríguez Gil, Alfonso
Fernández Arévalo, María Mercedes
Flores Mosquera, María
Chávez de Diego, Sebastián
Gañán-Calvo, Alfonso M.
|Department||Universidad de Sevilla. Departamento de Farmacia y Tecnología Farmacéutica
Universidad de Sevilla. Departamento de Genética
Universidad de Sevilla. Departamento de Ingeniería Aeroespacial y Mecánica de Fluidos
Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER)
|Abstract||Paramagnetic particles offer an extensive improvement in the magnetic separation or purification of a wide variety of protein molecules. Most commercial paramagnetic particles are synthesized by laborious and costly ...
Paramagnetic particles offer an extensive improvement in the magnetic separation or purification of a wide variety of protein molecules. Most commercial paramagnetic particles are synthesized by laborious and costly procedures. A straightforward production of paramagnetic microparticles with homogeneous and selectable sizes using flow focusing (FF) technology is described in this work. The development of an initial formulation of a stable iron oxide suspension compatible with the FF requirements is also reported. The obtained particles, below 10 microns in diameter and presenting smooth and reactive surface, were codified with an organic fluorophore and showed excellent properties for covalent attachment of biomolecules such as proteins and its subsequent recognition by flow cytometry. Furthermore, particles with suitable magnetite content resulted as well-suited for commercial magnet separators for these purposes.