dc.creator | Borrero de Acuña, José Manuel | es |
dc.creator | Poblete Castro, Ignacio | es |
dc.date.accessioned | 2024-03-14T16:05:11Z | |
dc.date.available | 2024-03-14T16:05:11Z | |
dc.date.issued | 2023-02 | |
dc.identifier.citation | Borrero de Acuña, J.M. y Poblete Castro, I. (2023). Rational engineering of natural polyhydroxyalkanoates producing microorganisms for improved synthesis and recovery. Microbial Biotechnology, 16 (2), 262-285. https://doi.org/10.1111/1751-7915.14109. | |
dc.identifier.issn | 1751-7907 | es |
dc.identifier.issn | 1751-7915 | es |
dc.identifier.uri | https://hdl.handle.net/11441/156281 | |
dc.description.abstract | Microbial production of biopolymers derived from renewable substrates and waste streams reduces our heavy reliance on petrochemical plastics. One of the most important biodegradable polymers is the family of polyhydroxyalkanoates (PHAs), naturally occurring intracellular polyoxoesters produced for decades by bacterial fermentation of sugars and fatty acids at the industrial scale. Despite the advances, PHA production still suffers from heavy costs associated with carbon substrates and downstream processing to recover the intracellular product, thus restricting market positioning. In recent years, model-aided metabolic engineering and novel synthetic biology approaches have spurred our understanding of carbon flux partitioning through competing pathways and cellular resource allocation during PHA synthesis, enabling the rational design of superior biopolymer producers and programmable cellular lytic systems. This review describes these attempts to rationally engineering the cellular operation of several microbes to elevate PHA production on specific substrates and waste products. We also delve into genome reduction, morphology, and redox cofactor engineering to boost PHA biosynthesis. Besides, we critically evaluate engineered bacterial strains in various fermentation modes in terms of PHA productivity and the period required for product recovery. | es |
dc.description.sponsorship | Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT) 1210332 | es |
dc.description.sponsorship | Agencia Nacional de Investigación y Desarrollo (ANID) INACH ACT192057, INACH RG_17_19 | es |
dc.description.sponsorship | Junta de Andalucía EMERGIA20_00048 | es |
dc.format | application/pdf | es |
dc.format.extent | 24 p. | es |
dc.language.iso | eng | es |
dc.publisher | Wiley-Blackwell | es |
dc.relation.ispartof | Microbial Biotechnology, 16 (2), 262-285. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Rational engineering of natural polyhydroxyalkanoates producing microorganisms for improved synthesis and recovery | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.relation.projectID | 1210332 | es |
dc.relation.projectID | INACH ACT192057 | es |
dc.relation.projectID | INACH RG_17_19 | es |
dc.relation.projectID | EMERGIA20_00048 | es |
dc.relation.publisherversion | https://doi.org/10.1111/1751-7915.14109 | es |
dc.identifier.doi | 10.1111/1751-7915.14109 | es |
dc.journaltitle | Microbial Biotechnology | es |
dc.publication.volumen | 16 | es |
dc.publication.issue | 2 | es |
dc.publication.initialPage | 262 | es |
dc.publication.endPage | 285 | es |
dc.contributor.funder | Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT). Chile | es |
dc.contributor.funder | Agencia Nacional de Investigación y Desarrollo (ANID). Chile | es |
dc.contributor.funder | Junta de Andalucía | es |