Show simple item record

Article

dc.creatorMartín Calvo, Paulaes
dc.creatorGarcía Hernández, Rosendo Migueles
dc.creatorRodríguez de la Cruz, Rosa Maríaes
dc.creatorPastor Loro, Ángel Manueles
dc.date.accessioned2023-11-27T16:15:40Z
dc.date.available2023-11-27T16:15:40Z
dc.date.issued2022
dc.identifier.citationMartín Calvo, P., García Hernández, R.M., Rodríguez de la Cruz, R.M. y Pastor Loro, Á.M. (2022). VEGF is an essential retrograde trophic factor for motoneurons. Proceedings of the National Academy of Sciences of the United States of America, 119 (26), e2202912119. https://doi.org/10.1073/pnas.2202912119.
dc.identifier.issn0027-8424es
dc.identifier.issn1091-6490es
dc.identifier.urihttps://hdl.handle.net/11441/151685
dc.description.abstractVEGF was initially discovered due to its angiogenic activity and therefore named "vascular endothelial growth factor."However, its more recently discovered neurotrophic activity may be evolutionarily more ancient. Our previous work showed that all the changes produced by axotomy on the firing activity and synaptic inputs of abducens motoneurons were completely restored after VEGF administration. Therefore, we hypothesized that the lack of VEGF delivered by retrograde transport from the periphery should also affect the physiology of otherwise intact abducens motoneurons. For VEGF retrograde blockade, we chronically applied a neutralizing VEGF antibody to the lateral rectus muscle. Recordings of extracellular single-unit activity and eye movements were made in alert cats before and after the application of the neutralizing antibody. Our data revealed that intact, noninjured abducens motoneurons retrogradely deprived of VEGF exhibited noticeable changes in their firing pattern. There is a general decrease in firing rate and a significant reduction in eye position and eye velocity sensitivity (i.e., a decrease in the tonic and phasic components of their discharge, respectively). Moreover, by means of confocal immunocytochemistry, motoneurons under VEGF blockade showed a marked reduction in the density of afferent synaptic terminals contacting with their cell bodies. Altogether, the present findings demonstrate that the lack of retrogradely delivered VEGF renders abducens motoneurons into an axotomy-like state. This indicates that VEGF is an essential retrograde factor for motoneuronal synaptic drive and discharge activity.es
dc.description.sponsorshipMinisterio de Ciencia e Innovación PGC2018-094654-B-100es
dc.description.sponsorshipJunta de Andalucía P20_00529, BIO-297es
dc.formatapplication/pdfes
dc.format.extent42 p.es
dc.language.isoenges
dc.publisherNational Academy of Scienceses
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America, 119 (26), e2202912119.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAbducenses
dc.subjectAxotomyes
dc.subjectNeurotrophic factorses
dc.subjectOculomotores
dc.subjectSynaptic strippinges
dc.titleVEGF is an essential retrograde trophic factor for motoneuronses
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/acceptedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Fisiologíaes
dc.relation.projectIDPGC2018-094654-B-100es
dc.relation.projectIDP20_00529es
dc.relation.projectIDBIO-297es
dc.relation.publisherversionhttps://doi.org/10.1073/pnas.2202912119es
dc.identifier.doi10.1073/pnas.2202912119es
dc.journaltitleProceedings of the National Academy of Sciences of the United States of Americaes
dc.publication.volumen119es
dc.publication.issue26es
dc.publication.initialPagee2202912119es
dc.contributor.funderMinisterio de Ciencia e Innovación (MICIN). Españaes
dc.contributor.funderJunta de Andalucíaes
dc.description.awardwinningPremio Anual Publicación Científica Destacada de la US. Facultad de Biología

FilesSizeFormatViewDescription
VEGF is an essential.pdf90.26MbIcon   [PDF] View/Open   Versión aceptada

This item appears in the following collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional