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dc.creatorLara Moreno, Albaes
dc.creatorRubio Bellido, Marinaes
dc.creatorMadrid Díaz, Fernandoes
dc.creatorVillaverde Capellán, Jaimees
dc.creatorSantos Morcillo, Juan Luises
dc.creatorAlonso Álvarez, Estebanes
dc.creatorMorillo González, Esmeraldaes
dc.identifier.citationLara Moreno, A., Aguilar-Romero, I., Rubio Bellido, M., Madrid Díaz, F., Villaverde Capellán, J., Santos Morcillo, J.L.,...,Morillo González, E. (2022). Novel nonylphenol-degrading bacterial strains isolated from sewage sludge: Application in bioremediation of sludge. Science of The Total Environment, 847 (157647).
dc.description.abstractNonylphenol (NP) is an anthropogenic pollutant frequently found in sewage sludge due to the insufficient degrading effectiveness of conventional WWTPs and has attracted attention as an endocrine disruptor. The aim of this study was to isolate specific NP-degrading bacteria from sewage sludge to be used in the degradation of this contaminant through bioaugmentation processes in aqueous solution and sewage sludge. Up to eight different bacterial strains were isolated, six of them not previously described as NP degraders. Bacillus safensis CN12 presented the best NP degradation in solution, and glucose used as an external carbon source increased its effect, reaching DT50 degradation values (time to decline to half the initial concentration of the pollutant) of only 0.9 days and a complete degradation in <7 days. Four NP metabolites were identified throughout the biodegradation process, showing higher toxicity than the parent contaminant. In sewage sludge suspensions, the endogenous microbiota was capable of partially degrading NP, but a part remained adsorbed as bound residue. Bioaugmentation was used for the first time to remove NP from sewage sludge to obtain more environmentally friendly biosolids. However, B. safensis CN12 was not able to degrade NP due to its high adsorption on sludge, but the use of a cyclodextrin (HPBCD) as availability enhancer allowed us to extract NP and degrade it in solution. The addition of glucose as an external carbon source gave the best results since the metabolism of the sludge microbiota was activated, and HPBCD was able to remove NP from sewage sludge to the solution to be degraded by B. safensis CN12. These results indicate that B. safensis CN12 can be used to degrade NP in water and sewage sludge, but the method must be improved using consortia of B. safensis CN12 with other bacterial strains able to degrade the toxic metabolites
dc.relation.ispartofScience of The Total Environment, 847 (157647).
dc.rightsAtribución 4.0 Internacional*
dc.subjectSewage sludgees
dc.subjectBacterial strainses
dc.subjectBacillus safensises
dc.titleNovel nonylphenol-degrading bacterial strains isolated from sewage sludge: Application in bioremediation of sludgees
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiología y Parasitologíaes
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Química Analíticaes
dc.contributor.groupUniversidad de Sevilla. FQM344: Análisis Químico Industrial y Medioambientales
dc.journaltitleScience of The Total Environmentes
dc.contributor.funderSpanish Ministry of Science, Innovation and Universities / AEI/FEDER, UE research project CTM2017-82472-C2-1-Res
dc.contributor.funderJunta de Andalucía (Council of Economic Transformation, Industry, Knowledge and University) / FEDER Andalucía PO 2014-2020 research project PY20_01069es

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Atribución 4.0 Internacional
Except where otherwise noted, this item's license is described as: Atribución 4.0 Internacional