Artículo
Pdx1 Is Transcriptionally Regulated by EGR-1 during Nitric Oxide-Induced Endoderm Differentiation of Mouse Embryonic Stem Cells
Autor/es | Salguero-Aranda, Carmen
Beltran Povea, Amparo Postigo-Corrales, Fátima Hitos, Ana Belén Díaz, Irene Caballano-Infantes, Estefanía Cahuana, Gladys M. |
Departamento | Universidad de Sevilla. Departamento de Citología e Histología Normal y Patológica |
Fecha de publicación | 2022 |
Fecha de depósito | 2022-10-26 |
Publicado en |
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Resumen | The transcription factor, early growth response-1 (EGR-1), is involved in the regulation of
cell differentiation, proliferation, and apoptosis in response to different stimuli. EGR-1 is described
to be involved in ... The transcription factor, early growth response-1 (EGR-1), is involved in the regulation of cell differentiation, proliferation, and apoptosis in response to different stimuli. EGR-1 is described to be involved in pancreatic endoderm differentiation, but the regulatory mechanisms controlling its action are not fully elucidated. Our previous investigation reported that exposure of mouse embryonic stem cells (mESCs) to the chemical nitric oxide (NO) donor diethylenetriamine nitric oxide adduct (DETA-NO) induces the expression of early differentiation genes such as pancreatic and duodenal homeobox 1 (Pdx1). We have also evidenced that Pdx1 expression is associated with the release of polycomb repressive complex 2 (PRC2) and P300 from the Pdx1 promoter; these events were accompanied by epigenetic changes to histones and site-specific changes in the DNA methylation. Here, we investigate the role of EGR-1 on Pdx1 regulation in mESCs. This study reveals that EGR-1 plays a negative role in Pdx1 expression and shows that the binding capacity of EGR-1 to the Pdx1 promoter depends on the methylation level of its DNA binding site and its acetylation state. These results suggest that targeting EGR-1 at early differentiation stages might be relevant for directing pluripotent cells into Pdx1-dependent cell lineages. |
Agencias financiadoras | Instituto de Salud Carlos III Consejería de Igualdad, Salud y Políticas Sociales, Junta de Andalucía Consejería de Economía, Innovación, Ciencia y Empleo, Junta de Andalucía European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) Servicio Andaluz de Salud |
Identificador del proyecto | FI11/00301
PI105/2010 TCMR06/009 PI-0022 CTS-7127/2011) |
Cita | Salguero-Aranda, C., Beltran Povea, A., Postigo-Corrales, F., Hitos, A.B., Díaz, I., Caballano-Infantes, E. y Cahuana, G.M. (2022). Pdx1 Is Transcriptionally Regulated by EGR-1 during Nitric Oxide-Induced Endoderm Differentiation of Mouse Embryonic Stem Cells. International Journal of Molecular Sciences, 23 (7), 3920. https://doi.org/10.3390/ijms23073920. |
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