Article
The nodD1 Gene of Sinorhizobium fredii HH103 Restores Nodulation Capacity on Bean in a Rhizobium tropici CIAT 899 nodD1/nodD2 Mutant, but the Secondary Symbiotic Regulators nolR, nodD2 or syrM Prevent HH103 to Nodulate with This Legume
Author | Fuentes Romero, Francisco
![]() ![]() ![]() ![]() ![]() Navarro Gómez, Pilar ![]() ![]() ![]() ![]() ![]() ![]() ![]() Ayala García, Paula ![]() ![]() ![]() Moyano Bravo, Isamar López Baena, Francisco Javier ![]() ![]() ![]() ![]() ![]() ![]() ![]() Pérez Montaño, Francisco de Asís ![]() ![]() ![]() ![]() ![]() ![]() ![]() Ollero Márquez, Francisco Javier ![]() ![]() ![]() ![]() ![]() ![]() ![]() Ollero Márquez, Francisco Javier ![]() ![]() ![]() ![]() ![]() ![]() ![]() Acosta Jurado, Sebastián Vinardell González, José María ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
Department | Universidad de Sevilla. Departamento de Microbiología |
Date | 2022 |
Published in |
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Abstract | Rhizobial NodD proteins and appropriate flavonoids induce rhizobial nodulation gene expression. In this study, we show that the nodD1 gene of Sinorhizobium fredii HH103, but not the nodD2 gene, can restore the nodulation ... Rhizobial NodD proteins and appropriate flavonoids induce rhizobial nodulation gene expression. In this study, we show that the nodD1 gene of Sinorhizobium fredii HH103, but not the nodD2 gene, can restore the nodulation capacity of a double nodD1/nodD2 mutant of Rhizobium tropici CIAT 899 in bean plants (Phaseolus vulgaris). S. fredii HH103 only induces pseudonodules in beans. We have also studied whether the mutation of different symbiotic regulatory genes may affect the symbiotic interaction of HH103 with beans: ttsI (the positive regulator of the symbiotic type 3 protein secretion system), and nodD2, nolR and syrM (all of them controlling the level of Nod factor production). Inactivation of either nodD2, nolR or syrM, but not that of ttsI, affected positively the symbiotic behavior of HH103 with beans, leading to the formation of colonized nodules. Acetylene reduction assays showed certain levels of nitrogenase activity that were higher in the case of the nodD2 and nolR mutants. Similar results have been previously obtained by our group with the model legume Lotus japonicus. Hence, the results obtained in the present work confirm that repression of Nod factor production, provided by either NodD2, NolR or SyrM, prevents HH103 to effectively nodulate several putative host plants. |
Funding agencies | Ministerio de Ciencia, Innovación y Universidades (MICINN). España |
Project ID. | PID2019-107634RB-I00
![]() AGL2016-77163-R ![]() |
Citation | Fuentes Romero, F., Navarro Gómez, P., Ayala García, P., Moyano Bravo, I., López Baena, F.J., Pérez Montaño, F.d.A.,...,Vinardell González, J.M. (2022). The nodD1 Gene of Sinorhizobium fredii HH103 Restores Nodulation Capacity on Bean in a Rhizobium tropici CIAT 899 nodD1/nodD2 Mutant, but the Secondary Symbiotic Regulators nolR, nodD2 or syrM Prevent HH103 to Nodulate with This Legume. Microorganisms, 10 (1), 139. |
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