Artículo
In vivo genotoxicity evaluation of cylindrospermopsin in rats using a combined micronucleus and comet assay
Autor/es | Díez-Quijada Jiménez, Leticia
Llana Ruiz-Cabello, María Catunescu, Giorgiana M. Puerto Rodríguez, María Moyano, Rosario Jos Gallego, Ángeles Mencía Cameán Fernández, Ana María |
Departamento | Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal |
Fecha de publicación | 2019 |
Fecha de depósito | 2021-12-20 |
Publicado en |
|
Resumen | Cylindrospermopsin (CYN) is a potent cyanotoxin recognized as an emerging human threat due to its cytotoxicity and potential carcinogenicity. Although the genotoxicity of CYN has been extensively studied in vitro, limited ... Cylindrospermopsin (CYN) is a potent cyanotoxin recognized as an emerging human threat due to its cytotoxicity and potential carcinogenicity. Although the genotoxicity of CYN has been extensively studied in vitro, limited data are available on its in vivo genotoxicity. The aim of this study was to evaluate the in vivo genotoxicity of pure CYN (7.5–75 μg/kg body weight) after oral exposure of rats through a combined assay of the micronucleus test (MN) in bone marrow, and the standard and modified comet assay in stomach, liver and blood. Also, histopathological changes in stomach and liver were evaluated. Positive results in the MN test were observed in bone marrow in the exposed rats at all the tested concentrations. However, the comet assay revealed that CYN did not induce DNA strand breaks nor oxidative DNA damage in any of the tissues investigated. Finally, histopathological changes were observed in stomach and liver (7.5–75 μg/kg) in intoxicated rats. These results could indicate that CYN is able to induce irritation in stomach before its biotransformation in rats orally exposed, and genotoxicity in bone marrow. |
Agencias financiadoras | Ministerio de Economía y Competitividad (MINECO). España European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) |
Identificador del proyecto | AGL2015-64558-R |
Cita | Díez-Quijada Jiménez, L., Llana Ruiz-Cabello, M., Catunescu, .M., Puerto Rodríguez, M., Moyano, R., Jos Gallego, Á.M. y Cameán Fernández, A.M. (2019). In vivo genotoxicity evaluation of cylindrospermopsin in rats using a combined micronucleus and comet assay. Food and Chemical Toxicology, 132, 110664. |
Ficheros | Tamaño | Formato | Ver | Descripción |
---|---|---|---|---|
10.1016_j.fct.2019.110664.pdf | 1.336Mb | [PDF] | Ver/ | Postprint |