Now showing items 1-10 of 16
Oxygen-sensitive calcium channels in vascular smooth muscle and their possible role in hypoxic arterial relaxation
We have investigated the modifications of cytosolic [Ca2+] and the activity of Ca2+ channels in freshly dispersed arterial myocytes to test whether lowering 02 tension (P02) directly influences Ca2+ homeostasis in these ...
Hypoxia induces voltage-dependent Ca2+ entry and quantal dopamine secretion in carotid body glomus cells
We have investigated the changes of cytosolic [Ca2+1 and the secretory -activity in single glomus cells dispersed from rabbit carotid bodies during exposure to solutions with variable 02 tension (Po2). In normoxic conditions ...
Oxygen sensing by ion channels and chemotransduction in single glomus cells
We have monitored cytosolic [Ca2+] and dopamine release in intact fura-2-loaded glomus cells with microfluoroimetry and a polarized carbon fiber electrode. Exposure to low PO2 produced a rise of cytosolic [Ca2+] with two ...
A Small Domain in the N Terminus of the Regulatory a-Subunit Kv2.3 Modulates Kv2.1 Potassium Channel Gating
Recent work has demonstrated the existence of regulatory K1 channel a-subunits that are electrically silent but capable of forming heterotetramers with other pore-forming subunits to modify their function. We have investigated ...
Gating of O2-sensitive K + Channels of Arterial Chemoreceptor Cells and Kinetic Modifications Induced by Low PO2
We have studied the kinetic properties of the O2-sensitive K+ channels (KO2 channels) of dissociated glomus cells from rabbit carotid bodies exposed to variable O2 tension (PO2). Experiments were done using single-channel ...
Identification and Functional Characterization of a K1 Channel a- Subunit with Regulatory Properties Specific to Brain
The physiological diversity of K1 channels mainly depends on the expression of several genes encoding different a-subunits. We have cloned a new K1 channel a-subunit (Kv2.3r) that is unable to form functional channels on ...
Sodium and Calcium Currents in Dispersed Mammalian Septal Neurons
Voltage-gated Na+ and Ca2+ conductances of freshly dissociated septal neurons were studied in the whole-cell configuration of the patch-clamp technique. All cells exhibited a large Na+ current with characteristic fast ...
Potassium Channel Types in Arterial Chemoreceptor Cells and Their Selective Modulation by Oxygen
Single K+ channel currents were recorded in excised membrane patches from dispersed chemoreceptor cells of the rabbit carotid body under conditions that abolish current flow through Na+ and Ca2+ channels. We have found ...