Now showing items 1-10 of 13
Increased neurotransmitter release at the neuromuscular junction in a mouse model of polyglutamine disease
In Huntington’s disease (HD), the expansion of polyglutamine (polyQ) repeats at the N terminus of the ubiquitous protein huntingtin (htt) leads to neurodegeneration in specific brain areas. Neurons degenerating in HD develop ...
Hypoxia induces voltage-dependent Ca2+ entry and quantal dopamine secretion in carotid body glomus cells
We have investigated the changes of cytosolic [Ca2+1 and the secretory -activity in single glomus cells dispersed from rabbit carotid bodies during exposure to solutions with variable 02 tension (Po2). In normoxic conditions ...
Oxygen sensing by ion channels and chemotransduction in single glomus cells
We have monitored cytosolic [Ca2+] and dopamine release in intact fura-2-loaded glomus cells with microfluoroimetry and a polarized carbon fiber electrode. Exposure to low PO2 produced a rise of cytosolic [Ca2+] with two ...
Structure/Function Analysis of Ca2 + Binding to the C2A Domain of Synaptotagmin 1
Synaptotagmin 1, a Ca2+ sensor for fast synaptic vesicle exocytosis, contains two C2 domains that form Ca2+ -dependent complexes with phospholipids. To examine the functional importance of Ca2+ binding to the C2A domain ...
Novel SCAMPs Lacking NPF Repeats: Ubiquitous and Synaptic Vesicle-Specific Forms Implicate SCAMPs in Multiple Membrane- Trafficking Functions
In vertebrates, secretory carrier membrane proteins (SCAMPs) 1–3 constitute a family of putative membrane-trafficking proteins composed of cytoplasmic N-terminal sequences with NPF repeats, four central transmembrane regions ...
Monitoring synaptic function at the neuromuscular junction of a mouse expressing synaptopHluorin
We monitored presynaptic exocytosis and vesicle recycling at neuromuscular junctions of transgenic mice expressing synaptopHluorin (spH), using simultaneous optical and electrophysiological recordings. Synaptic transmission ...
Deletion of CASK in mice is lethal and impairs synaptic function
CASK is an evolutionarily conserved multidomain protein composed of an N-terminal Ca2_/calmodulin-kinase domain, central PDZ and SH3 domains, and a C-terminal guanylate kinase domain. Many potential activities for CASK ...
CSP -deficiency causes massive and rapid photoreceptor degeneration
Cysteine string protein (CSP) _ is an abundant synaptic vesicle protein that contains a DNA-J domain characteristic of Hsp40-type cochaperones. Previous studies showed that deletion of CSP_ in mice leads to massive lethal ...
Cysteine String Protein- Prevents Activity-Dependent Degeneration in GABAergic Synapses
The continuous release of neurotransmitter could be seen to place a persistent burden on presynaptic proteins, one that could compromise nerve terminal function. This supposition and the molecular mechanisms that might ...
Examining synaptotagmin 1 function in dense core vesicle exocytosis under direct control of Ca2
We tested the long-standing hypothesis that synaptotagmin 1 is the Ca2 +_ sensor for fast neurosecretion by analyzing the intracellular Ca 2+ dependence of large dense-core vesicle exocytosis in a mouse strain carrying a ...