Now showing items 1-10 of 22
Regulatory elements located in the upstream region of the Rhizobium leguminosarum rosR global regulator are essential for its transcription and mRNA stability
(Multidisciplinary Digital Publishing Institute (MDPI), 2017)
Rhizobium leguminosarum bv. trifolii is a soil bacterium capable of establishing a symbiotic relationship with clover (Trifolium spp.). Previously, the rosR gene, encoding a global regulatory protein involved in motility, ...
A transcriptomic analysis of the effect of genistein on Sinorhizobium fredii HH103 reveals novel rhizobial genes putatively involved in symbiosis
(Nature Publishing Group, 2016)
Sinorhizobium fredii HH103 is a rhizobial soybean symbiont that exhibits an extremely broad host-range. Flavonoids exuded by legume roots induce the expression of rhizobial symbiotic genes and activate the bacterial protein ...
Structure and biological roles of Sinorhizobium fredii HH103 exopolysaccharide
(Public Library of Science, 2014)
Here we report that the structure of the Sinorhizobium fredii HH103 exopolysaccharide (EPS) is composed of glucose, galactose, glucuronic acid, pyruvic acid, in the ratios 5:2:2:1 and is partially acetylated. A S. fredii ...
Bacterial Molecular Signals in the Sinorhizobium fredii-Soybean Symbiosis
(MDPI AG, 2016)
Sinorhizobium (Ensifer) fredii (S. fredii) is a rhizobial species exhibiting a remarkably broad nodulation host-range. Thus, S. fredii is able to effectively nodulate dozens of different legumes, including plants forming ...
Regulation and symbiotic significance of nodulation outer proteins secretion in Sinorhizobium fredii HH103
In this work we show that the Sinorhizobium fredii HH103 ttsI gene is essential for the expression of the tts genes and secretion of nodulation outer proteins (Nops). Moreover, we demonstrate for the first time, to our ...
Transcriptomic studies of the effect of nod gene-inducing molecules in rhizobia: Different weapons, one purpose
(Multidisciplinary Digital Publishing Institute (MDPI), 2018)
Simultaneous quantification of transcripts of the whole bacterial genome allows the analysis of the global transcriptional response under changing conditions. RNA-seq and microarrays are the most used techniques to measure ...
Exopolysaccharide Production by Sinorhizobium fredii HH103 Is Repressed by Genistein in a NodD1-Dependent Manner
(Public Library of Science, 2016)
In the rhizobia-legume symbiotic interaction, bacterial surface polysaccharides, such as exopolysaccharide (EPS), lipopolysaccharide (LPS), K-antigen polysaccharide (KPS) or cyclic glucans (CG), appear to play crucial roles ...
The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population
(Nature Publishing Group, 2017)
The question of how genotypic and ecological units arise and spread in natural microbial populations remains controversial in the field of evolutionary biology. Here, we investigated the early stages of ecological and ...
A pyrF auxotrophic mutant of Sinorhizobium fredii HH103 impaired in its symbiotic interaction with soybean and other legumes
(Sociedad Española de Microbiología. Viguera Editores, 2007)
Transposon Tn5-Mob mutagenesis allowed the selection of a Sinorhizobium fredii HH103 mutant derivative (SVQ 292) that requires the presence of uracil to grow in minimal media. The mutated gene, pyrF, codes for an ...