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Artículo
Site-directed Mutagenesis of Cytochromec 6 from Synechocystissp. PCC 6803
(Elsevier, 1999)
This paper reports the first site-directed mutagenesis analysis of any cytochrome c 6, a heme protein that performs the same function as the copper-protein plastocyanin in the electron transport chain of photosynthetic ...
Artículo
Valores ecológicos de un bosque de rivera del entorno de Doñana
(Junta de Andalucía. Consejería de Medio Ambiente, 1999)
Los valores ecológicos y ambientales que presenta el Arroyo de Pilas favorecieron su inclusión en los límites del Parque Natural de Doñana en 1997 con la aprobación de Plan de Ordenación de Recursos Naturales de este ...
Artículo
Sobre la teoría y práctica de la ciencia
(CSIC, 1999)
La investigación y el desarrollo —normalmente conocidos por su abreviatura I+D—, así como la innovación tecnológica se consideran, sin lugar a dudas, los pilares básicos del progreso. Lo que no parece estar tan claro son ...
Artículo
Los elementos y moléculas de la vida
(Iberica: Actualidad Científica, 1999)
Artículo
Glutamine synthetase inactivation by protein-protein interaction
(National Academy of Sciences, 1999)
Glutamine synthetase (GS; EC 6.3.1.2) is the pivotal enzyme of nitrogen metabolism in prokaryotes. Control of bacterial GS activity by reversible adenylylation has provided one of the classical paradigms of signal transduction ...
Artículo
Oxidizing Side of the Cyanobacterial Photosystem I
(Elsevier, 1999)
Photosystem I (PSI) interacts with plastocyanin or cytochrome c 6 on the luminal side. To identify sites of interaction between plastocyanin/cytochromec 6 and the PSI core, site-directed mutations were generated in the ...
Artículo
The global nitrogen regulator NtcA regulates transcription of the signal transducer P(II) (GlnB) and influences its phosphorylation level in response to nitrogen and carbon supplies in the cyanobacterium Synechococcus sp. strain PCC 7942
(American Society for Microbiology, 1999)
The P(II) protein is encoded by a unique glnB gene in Synechococcus sp. strain PCC 7942. Its expression has been analyzed in the wild type and in NtcA-null mutant cells grown under different conditions of nitrogen and ...
Artículo
Site-directed Mutagenesis of Cytochromec 6 from Anabaena Species PCC 7119
(Elsevier, 1999)
A number of surface residues of cytochromec 6 from the cyanobacterium Anabaenasp. PCC 7119 have been modified by site-directed mutagenesis. Changes were made in six amino acids, two near the heme group (Val-25 and Lys-29) ...
Artículo
Cloning and expression of the algL gene, encoding the Azotobacter chroococcum alginate lyase: Purification and characterization of the enzyme
(American Society for Microbiology, 1999)
The alginate lyase-encoding gene (algL) of Azotobacter chroococcum was localized to a 3.1-kb EcoRI DNA fragment that revealed an open reading frame of 1,116 bp. This open reading frame encodes a protein of 42.98 kDa, in ...
Artículo
Substrate binding and catalysis by ribonuclease P from cyanobacteria and Escherichia coli are affected differently by the 3′ terminal CCA in tRNA precursors
(The National Academy of Sciences, 1999)
We have studied the effect of the 3′ terminal CCA sequence in precursors of tRNAs on catalysis by the RNase P RNA or the holoenzyme from the cyanobacterium Synechocystis sp. PCC 6803 in a completely homologous system. We ...