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dc.creatorCerro Sánchez, Pablo deles
dc.creatorAyala García, Paulaes
dc.creatorBuzón García, Pabloes
dc.creatorCastells Graells, Rogeres
dc.creatorLópez Baena, Francisco Javieres
dc.creatorOllero Márquez, Francisco Javieres
dc.creatorPérez Montaño, Francisco de Asíses
dc.date.accessioned2020-10-06T10:46:20Z
dc.date.available2020-10-06T10:46:20Z
dc.date.issued2020
dc.identifier.citationCerro Sánchez, P.d., Ayala García, P., Buzón García, P., Castells Graells, R., López Baena, F.J., Ollero Márquez, F.J. y Pérez Montaño, F.d.A. (2020). OnfD, an AraC-Type Transcriptional Regulator Encoded by Rhizobium tropici CIAT 899 and Involved in Nod Factor Synthesis and Symbiosis. Applied and Environmental Microbiology, 86 (19), e01297-1320.
dc.identifier.issn1098-5336es
dc.identifier.urihttps://hdl.handle.net/11441/101731
dc.description.abstractRhizobium tropici CIAT 899 is a broad-host-range rhizobial strain that establishes symbiotic interactions with legumes and tolerates different environmental stresses such as heat, acidity, or salinity. This rhizobial strain produces a wide variety of symbiotically active nodulation factors (NF) induced not only by the presence of plant-released flavonoids but also under osmotic stress conditions through the LysR-type transcriptional regulators NodD1 (flavonoids) and NodD2 (osmotic stress). However, the activation of NodD2 under high-osmotic-stress conditions remains elusive. Here, we have studied the role of a new AraC-type regulator (named as OnfD) in the symbiotic interaction of R. tropici CIAT 899 with Phaseolus vulgaris and Lotus plants. We determined that OnfD is required under salt stress conditions for the transcriptional activation of the nodulation genes and therefore the synthesis and export of NF, which are required for a successful symbiosis with P. vulgaris. Moreover, using bacterial two-hybrid analysis, we demonstrated that the OnfD and NodD2 proteins form homodimers and OnfD/NodD2 form heterodimers, which could be involved in the production of NF in the presence of osmotic stress conditions since both regulators are required for NF synthesis in the presence of salt. A structural model of OnfD is presented and discussed. IMPORTANCE The synthesis and export of rhizobial NF are mediated by a conserved group of LysR-type regulators, the NodD proteins. Here, we have demonstrated that a non-LysR-type regulator, an AraC-type protein, is required for the transcriptional activation of symbiotic genes and for the synthesis of symbiotically active NF under salt stress conditions.es
dc.description.sponsorshipEspaña Ministerio de Economía y Competitividad (project AGL2016-77163-R)es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherAmerican Society for Microbiologyes
dc.relation.ispartofApplied and Environmental Microbiology, 86 (19), e01297-1320.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAraC-type regulatorses
dc.subjectRhizobium tropicies
dc.subjectegumeses
dc.subjectplant-microbe interactionses
dc.subjectregulation of gene expressiones
dc.subjectsymbiosises
dc.subjecttranscriptional regulationes
dc.titleOnfD, an AraC-Type Transcriptional Regulator Encoded by Rhizobium tropici CIAT 899 and Involved in Nod Factor Synthesis and Symbiosises
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiologíaes
dc.relation.projectIDproject AGL2016-77163-Res
dc.relation.publisherversionhttp://dx.doi.org/10.1128/AEM.01297-20es
dc.identifier.doi10.1128/AEM.01297-20es
dc.journaltitleApplied and Environmental Microbiologyes
dc.publication.volumen86es
dc.publication.issue19es
dc.publication.initialPagee01297es
dc.publication.endPage1320es
dc.contributor.funderMinisterio de Economía y Competitividad (MINECO). Españaes

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