Artículos (Microbiología)
URI permanente para esta colecciónhttps://hdl.handle.net/11441/10904
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Examinando Artículos (Microbiología) por Premio "Premio Mensual Publicación Científica Destacada de la US. Facultad de Biología"
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Artículo Cisplatin-induced Cell Death Increases the degradation of the MRE11-RAD50-NBS1 Complex Through the Autophagy/lysosomal Pathway(Springer Nature, 2023) Belmonte Fernández, Alejandro; Herrero Ruiz, Joaquín; Galindo Moreno, María; Limón Mortés, María Cristina; Mora Santos, María del Mar; Sáez, Carmen; Japón, Miguel A.; Tortolero García, María Dolores; Romero Portillo, Francisco; Universidad de Sevilla. Departamento de Microbiología; Ministerio de Ciencia e Innovación (MICIN). España; Junta de AndalucíaCisplatin and other platinum-based anticancer agents are among the most widely used chemotherapy drugs in the treatment of different types of cancer. However, it is common to find patients who respond well to treatment at first but later relapse due to the appearance of resistance to cisplatin. Among the mechanisms responsible for this phenomenon is the increase in DNA damage repair. Here, we elucidate the effect of cisplatin on the MRN (MRE11-RAD50-NBS1) DNA damage sensor complex. We found that the tumor suppressor FBXW7 is a key factor in controlling the turnover of the MRN complex by inducing its degradation through lysosomes. Inhibition of lysosomal enzymes allowed the detection of the association of FBXW7-dependent ubiquitylated MRN with LC3 and the autophagy adaptor p62/SQSTM1 and the localization of MRN in lysosomes. Furthermore, cisplatin-induced cell death increased MRN degradation, suggesting that this complex is one of the targets that favor cell death. These findings open the possibility of using the induction of the degradation of the MRN complex after genotoxic damage as a potential therapeutic strategy to eliminate tumor cells.Artículo Knock-down of phosphoenolpyruvate carboxylase 3 negatively impacts growth, productivity, and responses to salt stress in sorghum (Sorghum bicolor L.)(Wiley, 2022) Osa Fernández, Clara de la; Pérez López, Jesús; Feria Bourrellier, Ana Belén; Baena Vaca, Guillermo; Marino, Daniel; Coleto, Inmaculada; Pérez Montaño, Francisco de Asís; Gandullo Tovar, Jacinto Manuel; Echevarría Ruiz de Vargas, Cristina; García-Mauriño Ruiz-Berdejo, Sofía; Monreal Hermoso, José Antonio; Universidad de Sevilla. Departamento de Biología Vegetal y Ecología; Universidad de Sevilla. Departamento de Microbiología; Junta de Andalucía; Gobierno Vasco; Ministerio de Economia, Industria y Competitividad (MINECO). EspañaPhosphoenolpyruvate carboxylase (PEPC) is a carboxylating enzyme with important roles in plant metabo-lism. Most studies in C4plants have focused on photosynthetic PEPC, but less is known about non-photosynthetic PEPC isozymes, especially with respect to their physiological functions. In this work, weanalyzed the precise roles of the sorghum (Sorghum bicolor) PPC3 isozyme by the use of knock-down lineswith the SbPPC3gene silenced (Ppc3lines).Ppc3plants showed reduced stomatal conductance and plantsize, a delay in flowering time, and reduced seed production. In addition, silenced plants accumulated stressindicators such as Asn, citrate, malate, and sucrose in roots and showed higher citrate synthase activity,even in control conditions. Salinity further affected stomatal conductance and yield and had a deeperimpact on central metabolism in silenced plants compared to wild type, more notably in roots, withPpc3plants showing higher nitrate reductase and NADH-glutamate synthase activity in roots and the accumula-tion of molecules with a higher N/C ratio. Taken together, our results show that although SbPPC3 is pre-dominantly a root protein, its absence causes deep changes in plant physiology and metabolism in rootsand leaves, negatively affecting maximal stomatal opening, growth, productivity, and stress responses insorghum plants. The consequences of SbPPC3silencing suggest that this protein, and maybe orthologs inother plants, could be an important target to improve plant growth, productivity, and resistance to saltstress and other stresses where non-photosynthetic PEPCs may be implicated.