Este archivo ha sido creado el 21-04-2025 por Pedro Valle-Romero GENERAL INFORMATION ------------------ 1. Dataset title: Research paper's dataset: “Conditions for vegetative propagation of three key halophytes from habitats of EU community interest: substrate nature, salinity and PGP-bacterial interactions and cost evaluation”. 2. Authorship: Name: Valle-Romero, Pedro Institution: Department of Plant Biology and Ecology, Faculty of Biology, University of Seville, 41012 (Seville, Spain) Email: pvalle@us.es ORCID: 0000-0002-3234-8840 Name: Martín-Peláez, M. Rocío Institution: Department of Plant Biology and Ecology, Faculty of Biology, University of Seville, 41012 (Seville, Spain) Email: mmartin10@us.es ORCID: 0000-0002-3234-8840 Name: Flores-Duarte, Noris J. Institution: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, 41012 (Seville, Spain) Email: nflores@us.es ORCID: 0000-0002-8887-5033 Name: Redondo-Gómez, Susana Institution: Department of Plant Biology and Ecology, Faculty of Biology, University of Seville, 41012 (Seville, Spain) Email: susana@us.es ORCID: 0000-0002-5280-9325 Name: Puglielli, Giacomo Institution: Department of Life Sciences, University of Trieste, Licio Giorgieri 10, 34127 (Trieste, Italy) Email: GIACOMO.PUGLIELLI@units.it ORCID: 0000-0003-0085-4535 Name: Zunzunegui, María Institution: Department of Plant Biology and Ecology, Faculty of Biology, University of Seville, 41012 (Seville, Spain) Email: zunzu@us.es ORCID: 0000-0002-3815-9290 Name: Álvarez-Cansino, Leonor Institution: Department of Plant Biology and Ecology, Faculty of Biology, University of Seville, 41012 (Seville, Spain) Email: leonor@us.es ORCID: 0000-0002-8346-303X Name: Rodríguez-Llorente, Ignacio D. Institution: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, 41012 (Seville, Spain) Email: irodri@us.es ORCID: 0000-0001-7621-8303 Name: Pajuelo, Eloísa Institution: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, 41012 (Seville, Spain) Email: epajuelo@us.es ORCID: 0000-0002-1084-5301 Name: Mateos-Naranjo, Enrique Institution: Department of Plant Biology and Ecology, Faculty of Biology, University of Seville, 41012 (Seville, Spain) Email: emana@us.es ORCID: 0000-0001-6276-5664 DESCRIPTION ---------- 1. Dataset language: Español 2. Abstract: The ecological transition requires expanding wetland restoration, given their importance in delivering key ecosystem services. Enhancing plant production for revegetation depends on evidence-based methods. This study aimed to optimize vegetative propagation techniques for three slow-growing perennial halophytes native to EU priority habitats: Atriplex portulacoides, Arthrocaulon macrostachyum, and Sporobolus maritimus. Cuttings were cultivated in three substrates (perlite, coconut fiber, and mulch), under two salinity conditions (0 and 171 mM NaCl), and with three inoculation treatments (control and two plant growth-promoting rhizobacteria consortia). Survival rates varied by species, with A. portulacoides achieving the highest (78.75 ± 18.75%), compared to A. macrostachyum and S. maritimus (57.50 ± 12.50% and 59.60 ± 9.00%, respectively). Coconut fiber promoted the highest survival, followed by perlite, regardless of inoculation. Inoculation particularly improved S. maritimus survival. Growth performance followed similar trends: cuttings in perlite and coconut fiber under saline conditions yielded greater biomass. Inoculation enhanced the growth of A. portulacoides and A. macrostachyum, as well as photosynthetic efficiency and carboxylation rates.A cost assessment revealed that this propagation method offers clear economic benefits over direct transplant approaches. These findings support the development of more efficient propagation strategies to supply plant material for future wetland restoration projects. 3. Keywords: Species, Substrate, Salinity, Inoculum 4. Date of data collection (fecha única o rango de fechas): 01-02-2022 – 30-05-2022 5. Publication Date: 21-04-2025 6. Grant information: Grant Agency: Ministerio de Ciencia e Innovación Grant Number: TED2021-131605B-I00 7. Geographical location/s of data collection: Marismas del Río Piedras y Odiel (Huelva, España) ACCESS INFORMATION ------------------------ 1. Creative Commons License of the dataset: CC-BY-NC 2. Dataset DOI: https://doi.org/10.12795/11441/172174 3. Related publication: Pedro Valle-Romero, M. Rocío Martín-Peláez, Noris J. Flores-Duarte, Susana Redondo-Gómez, Giacomo Puglielli, María Zunzunegui, Leonor Álvarez-Cansino, Ignacio D. Rodríguez-Llorente, Eloísa Pajuelo, Enrique Mateos-Naranjo, Conditions for vegetative propagation of three key halophytes from habitats of EU community interest: substrate nature, salinity and PGP-bacterial interactions and cost evaluation, Estuarine, Coastal and Shelf Science, Volume 320, 2025, 109315, ISSN 0272-7714, https://doi.org/10.1016/j.ecss.2025.109315. (https://www.sciencedirect.com/science/article/pii/S0272771425001933) VERSIONING AND PROVENANCE --------------- 1. Last modification date: 30-11-2024 2. Were data derived from another source?: No 3. Additional related data not included in this dataset: No METHODOLOGICAL INFORMATION ----------------------- Gas exchange analysis data were included using LI-COR 6800 measurements, where several parameters were recorded: net photosynthetic rate (AN), intercellular CO₂ concentration (Ci), stomatal conductance (gsw), intrinsic water use efficiency (iWUE), carboxylation rate (Ce), quantum efficiency of PSII (phiPSII), electron transport rate (ETR), and quantum efficiency of light use for CO₂ assimilation (phiCO₂). The maximum efficiency of photosystem II (Fv/Fm) was also measured using a fluorometer. Finally, at the end of the experiment, species survival was recorded. Plants were established with the treatments in the greenhouse on February 1, 2022, and all measurements were taken at the end of the experiment on May 30, 2025. 1. Description of the methods used to collect and generate the data: The protocol information and the corresponding bibliographic references supporting its selection are included in the officially published article. 2. Data processing methods: The published dataset was generated by processing and organizing the raw data collected during the experiment. Data were cleaned, standardized, and compiled into structured spreadsheets. Measurements from plant growth, gas exchange (using the LI-COR 6800), and fluorescence analyses were grouped by species, treatment, and sampling date. Only validated and quality-checked data were included in the final dataset. 3. Software or instruments needed to interpret the data: The data were organized in Excel (Microsoft) and statistically analyzed using R software version 4.3.1. 4. Information about instruments, calibration and standards: Gas exchange measurements were carried out using the LI-COR 6800 portable photosynthesis system (LI-COR Biosciences, Lincoln, NE, USA). The system was regularly calibrated according to the manufacturer's recommendations, including CO₂ and H₂O sensor calibration. Chlorophyll fluorescence was measured using a portable modulated fluorimeter (FMS, Hansatech Instruments), which was also calibrated before each measurement session using the provided dark-adaptation clips and reference settings. All instruments were operated under standardized environmental conditions and settings were kept consistent throughout the experiment. 5. Environmental or experimental conditions: Fully developed plants were collected in February 2022 from high, mid, and low-marsh zones in the Piedras River marshes (Huelva, Spain), and transplanted into pots with native soil in a greenhouse under controlled temperature, humidity, and natural light conditions. After a 5-day acclimation with regular watering, species-specific vegetative propagation methods were applied. Cuttings were planted in perforated 9 cm pots with different substrates and subjected to salinity and bacterial inoculation treatments for 90 days. Propagation involved 20 cm stem cuttings for A. macrostachyum, internode-based cuttings for A. portulacoides, and young shoots with roots for S. maritimus after initial rhizome trials failed. FILE OVERVIEW ---------------------- Dataset.csv 1. Explain the file naming conversion, si es aplicable: Only one file is included. 2. File list: File name: Dataset.csv Description: This is the only file that contains all the data: growth and photosynthetic parameters. 3. Relationship between files: Only one file is included. 4. File format: The file is in CSV format and is organized by data columns. Each parameter is listed in the first row, and the subsequent rows contain the results and each treatment. 5. If the dataset includes multiple files, specify the directory structure and relationships between the files: Only one file is included. SPECIFIC INFORMATION FOR TABULAR DATA ------------------------------------------- 1. Name file: Dataset.csv 2. Number of rows and columns: Number of rows: 1043 Number of columns: 29 3. Variables list: Especie, Sustrato, Salinidad, Inoculo, Long.raiz, Baerea, Braiz, SRL, Btotal, An, Ci, gsw, gsw_100, iWUE, Ce, Ce_1000, PhiPS2, PhiPS2_100, ETR, PhiCO2, PhiCO2_1000, FvFm, survival Variable name: Especie Description: Name of the plant species used (Atriplex portulacoides, Arthrocnemum macrostachyum, or Spartina maritima). Units of measure or value labels | Unidades de medida o etiquetas de valor: No unit of measurement. Variable name: Sustrato, Description: Name of the substrates or growth media used (Mulch "Mantillo", Perlite "Perlita or Coconut fiber "Fibra de coco"). Units of measure or value labels | Unidades de medida o etiquetas de valor: No unit of measurement. Variable name: Salinidad Description: Level of the salinity applied (0 mM or 171 mM NaCl). Units of measure or value labels | Unidades de medida o etiquetas de valor: Millimolar (mM) Variable name: Inoculo Description: Bacterial consortium used in the inoculum (no bacteria, inoculum 1, or inoculum 2). The bacterial strains are specified in the article. Units of measure or value labels | Unidades de medida o etiquetas de valor: No unit of measurement. Variable name: Long.raiz Description: Root length measurement. Units of measure or value labels | Unidades de medida o etiquetas de valor: cm Variable name: Baerea Description: Aboveground biomass (dry weight of the plant's aerial part). Units of measure or value labels | Unidades de medida o etiquetas de valor: g Variable name: Braiz Description: Belowground biomass (dry weight of the plant's root part). Units of measure or value labels | Unidades de medida o etiquetas de valor: g Variable name: SRL Description: Root length ratio (the proportion of root length relative to its biomass). Units of measure or value labels | Unidades de medida o etiquetas de valor: cm/mg Variable name: Btotal Description: Total biomass (dry weight of the plant). Units of measure or value labels | Unidades de medida o etiquetas de valor: cm/mg Variable name: An Description: Net photosynthetic rate Units of measure or value labels | Unidades de medida o etiquetas de valor: µmol CO₂ m⁻² s⁻¹ Variable name: Ci Description: Intercellular CO₂ concentration Units of measure or value labels | Unidades de medida o etiquetas de valor: µmol CO₂ mol⁻¹ Variable name: gsw Description: stomatal conductance Units of measure or value labels | Unidades de medida o etiquetas de valor: mol H₂O m⁻² s⁻¹ Variable name: iWUE Description: intrinsic water use efficiency Units of measure or value labels | Unidades de medida o etiquetas de valor: µmol CO₂ / mol H₂O Variable name: Ce Description: carboxylation rate Units of measure or value labels | Unidades de medida o etiquetas de valor: µmol CO₂ m⁻² s⁻¹ Variable name: PhiPS2 Description: quantum efficiency of PSII Units of measure or value labels | Unidades de medida o etiquetas de valor: No unit of measurement. Variable name: ETR Description: electron transport rate Units of measure or value labels | Unidades de medida o etiquetas de valor: µmol electrons m⁻² s⁻¹ Variable name: PhiCO2 Description: quantum efficiency of light use for CO₂ assimilation Units of measure or value labels | Unidades de medida o etiquetas de valor: mol CO₂ mol⁻¹ photons. Variable name: FvFm Description: the maximum photochemical efficiency of Photosystem II Units of measure or value labels | Unidades de medida o etiquetas de valor: No unit of measurement. Variable name: survival Description: survival of species after treatments period. Units of measure or value labels | Unidades de medida o etiquetas de valor: Percentage.