Este archivo ha sido creado el 21-12-2023 por Ana Belén Cerezo López
 

GENERAL INFORMATION
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1. Dataset title: Anti-VEGF Effect of Bioactive Indolic Compounds and Hydroxytyrosol Metabolites [Dataset]



2. Authorship:

	Name: Gallardo-Fernández, Marta
	Institution: Universidad de Sevilla
	Email:mgfernandez@us.es
	ORCID: https://orcid.org/0000-0001-7864-3506  

	Name: Cerezo, Ana B.
	Institution: Universidad de Sevilla
	Email: acerezo@us.es
	ORCID:https://orcid.org/0000-0001-9290-2535

	Name: Hornedo-Ortega, Ruth
	Institution: Universidad de Sevilla
	Email: rhornedo@us.es
	ORCID: https://orcid.org/0000-0003-1898-8806

	Name: Troncoso, Ana M.
	Institution: Universidad de Sevilla
	Email: amtroncoso@us.es
	ORCID: https://orcid.org/0000-0003-0291-2823

	Name: Garcia-Parrilla, M. Carmen
	Institution: Universidad de Sevilla
	Email: mcparrilla@us.es
	ORCID: https://orcid.org/0000-0002-0436-2784

DESCRIPTION
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1. Dataset language: English and Spanish



2. Abstract: The dasaset include the raw data of the western blot and ELISA experiments carried out in the paper entitled "Anti-VEGF Effect of Bioactive Indolic Compounds and Hydroxytyrosol Metabolites". Media and standard deviations are also included.
 


3. Keywords: Western blot, ELISA, VEGF



4. Date of data collection (fecha única o rango de fechas): from 26/11/2020 to 30/04/2021





ACCESS INFORMATION
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1. Creative Commons License of the dataset: Licencia CC-BY  



2. Dataset DOI: https://doi.org/10.12795/11441/152721



3. Related publication:This is the dataset of the following paper already published: Anti-VEGF Effect of Bioactive Indolic Compounds and Hydroxytyrosol Metabolites. Foods 2022, 11(4), 526; https://doi.org/10.3390/foods11040526





	
VERSIONING AND PROVENANCE
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2. Were data derived from another source?: NO







METHODOLOGICAL INFORMATION
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1. Description of the methods used to collect and generate the data:
Phosphorilated VEGFR-2 (ELISA Assay)
To measure the phosphorylated VEGFR-2 levels in the lysates, the sandwich ELISA kit
for phospho-VEGFR-2 (Tyr1175) was used following the manufacturer’s instruction.

Western Blot Analysis for PLCg1, Akt and eNOS
The protein lysates (26.8 microg) were mixed with NuPAGE lithium dodecyl sulfate (LDS)
sample buffer, NuPAGE DTT, before heating for 10 min at 70 ºC to denature proteins.
Subsequently, electrophoresis was performed in NuPAGE 4–12% Bis-Tris gels before being
transferred to 0.2 m nitrocellulose membranes. Tris buffered saline with Tween 20 (TBST)
was mixed with bovine serum albumin (BSA) to a final concentration of 5% (w/v) for blotting
of the membranes before incubation with primary antibodies (p-PLC1, PLC1, p-Akt, Akt,
p-eNOS, eNOS) overnight at 4 ºC. Subsequently, secondary antibody (anti-rabbit IgG-HRP)
in TBST + BSA (5%, w/v) was added to the membranes for 1 h at room temperature.
The bands were visualized using a SuperSignal West Pico chemiluminescent substrate
on an Amersham Imager 600 station (GE Healthcare live sciences, Marlborough, MA, USA).
Samples were analysed in duplicate, and the assay was performed three times.


3. Software or instruments needed to interpret the data: Excel spreadsheet





FILE OVERVIEW 
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1. Explain the file naming conversion, si es aplicable: Methodology used and compound name


2. File list:


	File name: ELISA indole piruvic acid different concentrations.xlsx
	Description:    raw data from the ELISA assay for VEGFR2 phosphorilation values of indole piruvic acid compound

	File name: ELISA ELISA DOPAL_MOPAL_MOPET.xlsx
	Description:    raw data from the ELISA assay for VEGFR2 phosphorilation values of DOPAL (3,4-dihydroxyphenylacetaldehyde) MOPAL, and MOPET compounds at different concentrations.

	File name: ELISA DOPAL_IPA_ILA_IBA.xlsx
	Description:    raw data from the ELISA assay for VEGFR2 phosphorilation values of DOPAL (3,4-dihydroxyphenylacetaldehyde), IPA (indole propionic acid), indole lactic acid (ILA), and IBA (indole butyric acid) at different concentrations.

	File name: ELISA indole piruvic acid 1 mM.xlsx
	Description:    raw data from the ELISA assay for VEGFR2 phosphorilation values of indole piruvic acid at 1 mM.

	File name: ELISA indole piruvic acid 0.1 mM.xlsx
	Description:    raw data from the ELISA assay for VEGFR2 phosphorilation values of indole piruvic acid at 0.1 mM.

	File name: Ratios Western Blot DOPAL.xlsx
	Description:    Ratios values (phosphoritated/total proteins) of the western blot assay for DOPAL (3,4-dihydroxyphenylacetaldehyde) compound for PLCgamma1, AKT and eNOS proteins. 

	File name: Ratios Western Blot indole piruvic acid.xlsx
	Description:    Ratios values (phosphoritated/total proteins) of the western blot assay for indole piruvic acid compound for PLCgamma1, AKT and eNOS proteins. 





4. File format:Excel spreadsheet .xlsx