Este archivo ha sido creado el 05-12-2023 por María Puerto 
 

GENERAL INFORMATION
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1. Dataset title:Study the immunotoxic effect of Cylindrospermopsin [Dataset]


2. Authorship:

	Name: Antonio Casas-Rodríguez
	Institution:Universidad de Sevilla
	Email: acasasr@us.es
	ORCID: 0000-0002-8590-4820
	
	Name:Óscar Cebadero-Domínguez 
	Institution:Universidad de Sevilla
	Email: ocebadero@us.es
	ORCID:  0000-0001-9481-562X

	
	Name:María Puerto
	Institution:Universidad de Sevilla
	Email: mariapuerto@us.es
	ORCID: 0000-0003-3476-7456

	Name:Ana M. Cameán
	Institution:Universidad de Sevilla
	Email: camean@us.es
	ORCID: 0000-0003-1524-748X
	
	Name:Ángeles Jos
	Institution:Universidad de Sevilla
	Email: angelesjos@us.es
	ORCID: 0000-0001-7546-3294


DESCRIPTION
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1. Dataset language: English



2. Abstract:

The cytotoxicity response of cylindrospermopsin (CYN) was investigated in monocytes (THP-1) and human T cells (Jurkat). CYN reduced cell viability, leading to mean effective concentrations
(EC50 24 h) of 6.00  ± 1.04 μM and 5.20 ± 1.20 μM for THP-1 and Jurkat cells, respectively, and induced cell death mainly by apoptosis in both experimental models. Moreover, CYN decreased the
differentiation of monocytes to macrophages after 48 h of exposure. In addition, an up-regulation of the mRNA expression of different cytokines, such as interleukin (IL) 2, IL-8, tumor necrosis
factor-alpha and interferon-gamma, was also observed mainly after 24 h exposure in both cell lines. However, only an increase the tumor necrosis factor-alpha in THP-1 supernatants was observed by ELISA.
Dataset contains the numerical values of the results obtained regarding cytotoxicity, differentiation, gene expression and interleukin leakage

3. Keywords: 
cylindrospermopsin, Immunotoxicity, THP-1 cells and Jurkat cells

4. Date of data collection: 10-01-2022
 

5. Publication Date: 20-12-2023



6. Grant information:

	Grant Agency: Spanish Ministerio de Ciencia e Innovación
	Grant Number: PID 2019-104890RB




ACCESS INFORMATION
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1. Creative Commons License of the dataset: CC-BY


2. Dataset DOI: https://doi.org/10.12795/11441/152718

3. Related publication:
Casas-Rodríguez, A., Cebadero-Dominguez, Ó., Puerto, M., Cameán, A. M., & Jos, A. (2023). 
Immunomodulatory Effects of Cylindrospermopsin in Human T Cells and Monocytes. Toxins, 15(4), 301. 
https://doi.org/10.3390/toxins15040301


VERSIONING AND PROVENANCE
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1. Last modification date: 01-03-2023

2. Were data derived from another source?: No


METHODOLOGICAL INFORMATION
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1. Description of the methods used to collect and generate the data:

The cytotoxicity data were obtained by the MTS reduction assay in THP-1 and Jurkat cells (Houtman et al., 2014). Differentiation experiments were based on the different adherent behaviour of monocytes and macrophages, 
performing in both populations the MTS assay (Muller et al.,2007). For flow cytometry, the cells were incubated in anexin V-FITC conjugate and Propidium Iodine. Gene expression was assayed by RT-PCR in both cells and interleukin leakage by ELISA (THP-1 cells). 
References:
Houtman, J., Maisanaba, S., Puerto, M., Gutierrez-Praena, D., Jord´a, M., Aucejo, S.,Jos, A., 2014. Toxicity assessment of organomodified clays used in food contact materials on human target cell lines. Appl. Clay Sci. 90, 150–158. https://doi.org/
10.1016/j.clay.2014.01.009.
Park, E.K., Jung, H.S., Yang, H.I., Yoo, M.C., Kim, C., Kim, K.S., 2007. Optimized THP-1 differentiation is required for the detection of responses to weak stimuli. Inflamm.
Res. 56 (1), 45–50. https://doi.org/10.1007/s00011-007-6115-5.

2. Data processing methods:
For cytotoxicity and differentiation data, from absorbance measurements mean and sd values were generated and values were expressed in % of the control. 
For flow cytometry, the cell´s fluorescence was determinated using MACSQuant VYB citometer.
For gene expression analysis, the LightCycler®480 System was used to amplification. The 2-ΔΔCT method was used to determine the results.
The levels of different cytokines (IL-1β, IL-2, IL-6, TNF-α, IFN-γ) were measured using the Bio-Plex Pro Human Chemokine Assays (Cat:171304090 M), following the manufacturer’s instructions (Bio-Rad Laboratories, Inc., Hercules, California, USA

3. Software or instruments needed to interpret the data: Graph-Pad Prism 9 version 9.0.0 software.

6. Quality-assurance procedures performed on the data: Negative and positive controls were included.


FILE OVERVIEW 
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2. File list:

	File name:Study the immunotoxic effect of cylindrospermopsin [Dataset]
	Description: The dataset is a excel file with 6 sheets with the raw data of Citotoxicity, Differentiation, Flow cytometry, PCR Jurkat, PCR THP-1, ELISA   



4. File format:Excel


MORE INFORMATION
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