Este archivo ha sido creado el 29-11-2023 por Angeles Jos
 

GENERAL INFORMATION
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1. Dataset title:Study the immunotoxic effect of reduced graphene [Dataset]


2. Authorship:

	Name:Óscar Cebadero-Domínguez 
	Institution:Universidad de Sevilla
	Email: ocebadero@us.es
	ORCID:  0000-0001-9481-562X

	Name: Antonio Casas-Rodríguez
	Institution:Universidad de Sevilla
	Email: acasasr@us.es
	ORCID: 0000-0002-8590-4820

	Name:María Puerto
	Institution:Universidad de Sevilla
	Email: mariapuerto@us.es
	ORCID: 0000-0003-3476-7456

	Name:Ana M. Cameán
	Institution:Universidad de Sevilla
	Email: camean@us.es
	ORCID: 0000-0003-1524-748X
	
	Name:Ángeles Jos
	Institution:Universidad de Sevilla
	Email: angelesjos@us.es
	ORCID: 0000-0001-7546-3294


DESCRIPTION
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1. Dataset language: English



2. Abstract:

The cytotoxicity response of reduced graphene oxide (rGO) was investigated in monocytes (THP-1) and human T cells (Jurkat). A mean effective concentration (EC50-24 h) of 121.45 ± 11.39 μg/mL and 207.51 ± 21.67 μg/mL for cytotoxicity was obtained in THP-1 and Jurkat cells, respectively. 
rGO decreased THP-1 monocytes differentiation at the highest concentration after 48 h of exposure. Regarding the inflammatory response at genetic level, changes (up and downregulations) were observed depending on the cell line,
 the exposure time and the interleukins investigated. Apoptosis/ necrosis genes expression was not altered in THP-1 cells but in Jurkat cells BAX and BCL-2 were downregulated after 4h. 
Finally, rGO did not trigger a significant release of any cytokine at any exposure time assayed. Dataset contains the numerical values of the results obtained regarding cytotoxicity, differentiation, gene expression and interleukin leakage

3. Keywords: 
Reduced graphene oxide, Immunotoxicity, cytotoxicity, differentiation, RT-PCR, interleukins

4. Date of data collection: 10-01-2022
 

5. Publication Date: 20-12-2023



6. Grant information:

	Grant Agency: Fondo Europeo de Desarrollo Regional (FEDER) y Consejería de Economía, Conocimiento, Empresas y Universidad de la Junta de Andalucía, Programa Operativo FEDER 2014–2020
	Grant Number: US-1259106

	Grant Agency: PAIDI-2020/FEDER, Consejería de transformación  Económica, Industria, Conocimiento y Universidades, Junta de Andalucía
	Grant Number: P18-RT-1993





ACCESS INFORMATION
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1. Creative Commons License of the dataset: CC-BY


2. Dataset DOI: https://doi.org/10.12795/11441/152721

3. Related publication:

Cebadero-Domínguez O, Casas-Rodríguez A, Puerto M, Cameán AM, Jos A. In vitro safety assessment of reduced graphene oxide in human monocytes and T cells. Environmental Research 232 (2023) 116356. https://doi.org/10.1016/j.envres.2023.116356



VERSIONING AND PROVENANCE
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1. Last modification date: 23-05-2023

2. Were data derived from another source?: No


METHODOLOGICAL INFORMATION
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1. Description of the methods used to collect and generate the data:

The cytotoxicity data were obtained by the MTS reduction assay in THP-1 and Jurkat cells (Houtman et al., 2014). Differentiation experiments were based on the different adherent behaviour of monocytes and macrophages, 
performing in both populations the MTS assay (Muller et al.,2007). Gene expression was assayed by RT-PCR in both cells and interleukin leakage by ELISA (THP-1 cells). 
References:
Houtman, J., Maisanaba, S., Puerto, M., Guti´errez-Praena, D., Jord´a, M., Aucejo, S.,Jos, A., 2014. Toxicity assessment of organomodified clays used in food contact materials on human target cell lines. Appl. Clay Sci. 90, 150–158. https://doi.org/
10.1016/j.clay.2014.01.009.
Park, E.K., Jung, H.S., Yang, H.I., Yoo, M.C., Kim, C., Kim, K.S., 2007. Optimized THP-1 differentiation is required for the detection of responses to weak stimuli. Inflamm.
Res. 56 (1), 45–50. https://doi.org/10.1007/s00011-007-6115-5.

2. Data processing methods:
For cytotoxicity and differentiation data, from absorbance measurements mean and sd values were generated and values were expressed in % of the control. 
For gene expression analysis, the LightCycler®480 System was used to amplification. The 2-ΔΔCT method was used to determine the results.
The levels of different cytokines (IL-1β, IL-2, IL-6, TNF-α, IFN-γ) were measured using the Bio-Plex Pro Human Chemokine Assays (Cat:171304090 M), following the manufacturer’s instructions (Bio-Rad Laboratories, Inc., Hercules, California, USA

3. Software or instruments needed to interpret the data: Graph-Pad Prism 9 version 9.0.0 software.

6. Quality-assurance procedures performed on the data: Negative and positive controls were included.


FILE OVERVIEW 
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2. File list:

	File name:Cebadero et al., 2023 Chemic-Biol Interact 372 110367 [dataset]
	Description: The dataset is a excel file with 5 sheets with the raw data of Citotoxicity, Differentiation, PCR THP-1, PCR Jurkat and ELISA   



4. File format:Excel


MORE INFORMATION
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