Este archivo ha sido creado el 18.01.2025 por Matilde Durán Lobato GENERAL INFORMATION ------------------ 1. Dataset title: Dataset from Engineering of Δ9-tetrahydrocannabinol delivery systems based on surface modified-PLGA nanoplatforms. Colloids and Surfaces B: Biointerfaces, 2014, vol. 123, pp. 114–122 (v.0) 2. Authorship: [ Obligatorio | Ponga la información de todos los autores siguiendo el suiguiente formato. Repita el esquema para cada autor.] Lucía Martín-Banderas Universidad de Sevilla ORCID: 0000-0003-1447-6125 Email: luciamartin@us.es Inmaculada Muñoz-Rubio Universidad de Sevilla ORCID: [Not available] Email: imunozrubio@us.es Josefa Álvarez-Fuentes Universidad de Sevilla ORCID: 0000-0002-3525-8488 Email: jalfuentes@us.es Matilde Durán-Lobato Universidad de Sevilla ORCID: 0000-0001-5534-6955 Email: mduran@us.es José L. Arias Universidad de Granada ORCID: 0000-0002-3437-3791 Email: jlarias@ugr.es Mª Ángeles Holgado Universidad de Sevilla ORCID: 0000-0002-3851-3405 Email: holgado@us.es Mercedes Fernández-Arévalo Universidad de Sevilla ORCID: 0000-0002-4283-3356 Email: mfarevalo@us.es DESCRIPTION ---------- 1. Dataset language: English 2. Abstract: This dataset corresponds to the article "Engineering of Δ9-tetrahydrocannabinol delivery systems based on surface modified-PLGA nanoplatforms." It includes data on the formulation and characterization of Δ9-THC-loaded PLGA nanoparticles (NPs) prepared via nanoprecipitation. Surface modifications include PEG, chitosan and PEG-chitosan coatings. The dataset encompasses particle size, zeta potential, encapsulation efficiency, drug loading, in vitro drug release profiles, cellular uptake (Caco-2 cells), and cytotoxicity assays. 3. Keywords: Blood compatibility; Cannabinoids; Cellular uptake; Cytotoxicity; PLGA nanoparticles; Surface functionalization; 9 -Tetrahydrocannabinol Compatibilidad sanguínea; Cannabinoides; Captación celular; Citotoxicidad; Nanopartículas de PLGA; Funcionalización de superficie; Δ9-Tetrahidrocannabinol 4. Date of data collection (fecha única o rango de fechas): 01.01.2013 – 31.07.2014 5. Publication Date: 16-09-2014 6. Grant information: [ Obligatorio si aplicable | Financiación recibida. Repita el esquema para cada organismo financiador.] Grant Agency: Instituto de Salud Carlos III Grant Number: FIS 11/02571 Grant Agency: Junta de Andalucía Grant Number: Project P09-CTS-5029 7. Geographical location/s of data collection: Facultad de Farmacia. Prof García González, 2, 41012 Sevilla, Andalucia, España Campus Reina Mercedes Coordinates: 37°21′39″N 5°59′18″O / 37.36094167, -5.98836111 ACCESS INFORMATION ------------------------ 1. Creative Commons License of the dataset: CC_BY 2. Dataset DOI: 3. Related publication: Martín-Banderas, L., Muñoz-Rubio, I., Álvarez-Fuentes, J., Durán-Lobato, M., Arias, J. L., Holgado, M. Á., & Fernández-Arévalo, M. (2014). Engineering of Δ9-tetrahydrocannabinol delivery systems based on surface modified-PLGA nanoplatforms. Colloids and Surfaces B: Biointerfaces, 123, 114–122. DOI: 10.1016/j.colsurfb.2014.09.002 4. Link to related datasets: VERSIONING AND PROVENANCE --------------- 1. Last modification date: 18-01-2025 2. Were data derived from another source?: NO 3. Additional related data not included in this dataset: METHODOLOGICAL INFORMATION ----------------------- 1. Description of the methods used to collect and generate the data: The dataset includes data from the formulation, characterization, and evaluation of Δ9-THC-loaded PLGA nanoparticles (NPs) prepared via nanoprecipitation. Surface modifications with PEG, chitosan, and PEG-chitosan were incorporated to enhance mucoadhesion and cellular uptake. Characterization data include particle size, zeta potential, drug loading, encapsulation efficiency, and in vitro drug release profiles under simulated gastric and intestinal conditions. Cellular uptake was studied using Caco-2 cells as a model for the gastrointestinal barrier, and cytotoxicity was assessed through MTT assays. Blood compatibility was evaluated by hemolysis and platelet activation tests. 2. Data processing methods: SPSS was used for statistical analyses, including Student’s t-test and one-way ANOVA to determine significance (p < 0.05). Drug quantification was performed using validated HPLC analysis, and cellular uptake was analyzed via flow cytometry. 3. Software or instruments needed to interpret the data: N/A 4. Information about instruments, calibration and standards: Nanoparticle Formulation: Nanoparticles were synthesized via nanoprecipitation using a syringe pump (Harvard Apparatus, UK) and lyophilized with a Cryodos freeze-drier (Telstar Industrial, Spain). Surface modifications were applied by incubation in respective PEG and chitosan solutions. Physicochemical Characterization: Size and Zeta Potential: Size distribution and zeta potential were measured using a Malvern Zetamaster 3000 (UK), calibrated with polystyrene latex standards. Morphology: Transmission electron microscopy (TEM) was performed using a Philips CM10 microscope (Netherlands). Drug Encapsulation and Release Studies: Encapsulation efficiency and drug loading were analyzed by HPLC using a Hitachi LaChrom® system with a diode array UV-Vis detector. Drug release was performed under controlled pH and temperature using a Unitronic orbital shaker (Selecta, Spain), and samples were analyzed by HPLC. Cytotoxicity Assays: MTT assays were conducted to evaluate cell viability on Caco-2 cells. Cells were incubated with nanoparticles for varying durations, followed by addition of MTT reagent and absorbance measurement at 540 nm using a BioTek Synergy HT microplate reader (USA). Hemocompatibility Studies: Hemolysis was determined by UV spectrophotometry (545 nm) after incubation of NPs with red blood cells. Platelet activation was assessed via sP-selectin quantification using ELISA methods. 5. Environmental or experimental conditions: N/A 6. Quality-assurance procedures performed on the data: [Opcional] FILE OVERVIEW ---------------------- [Se han de mencionar todos los archivos incluidos en el conjunto de datos, con el nombre y la extensión (.csv, .pdf, etc.) de cada archivo. Incluya la estructura de directorios]. 1. Explain the file naming conversion, si es aplicable: A single Excel file with each sheet collecting the data corresponding to a figure or table: -Figure 1: Data corresponding to manuscript Figure 1 on NPs physicochemical characterization. -Figure 3: Data corresponding to manuscript Figure 3 on the release profile of the different formulations at pH 2 and 7.4. -Figure 4: Data corresponding to manuscript Figure 4 on cell uptake of PLGA, PEG-PLGA and PEG-CS-PLGA NPs. -Figure 5: Data corresponding to manuscript Figure 5 on the cytotoxic effect on Caco-2 cells of free 9-THC, plain PLGA THC-loaded NPs, PEG-PLGA THC-loaded NPs, and PEG-CS-PLGA THC-loaded NPs. 2. File list: File name: Dataset from Engineering of THC delivery systems based on surface modified PLGA nanoplatforms. Description: A single Excel file with each sheet collecting the data corresponding to a figure or table: -Figure 1: Data corresponding to manuscript Figure 1 on NPs physicochemical characterization. -Figure 3: Data corresponding to manuscript Figure 3 on the release profile of the different formulations at pH 2 and 7.4. -Figure 4: Data corresponding to manuscript Figure 4 on cell uptake of PLGA, PEG-PLGA and PEG-CS-PLGA NPs. -Figure 5: Data corresponding to manuscript Figure 5 on the cytotoxic effect on Caco-2 cells of free 9-THC, plain PLGA THC-loaded NPs, PEG-PLGA THC-loaded NPs, and PEG-CS-PLGA THC-loaded NPs. 3. Relationship between files: [ Obligatorio si es aplicable | Relación entre los archivos] 4. File format: Excel 5. If the dataset includes multiple files, specify the directory structure and relationships between the files: [ Obligatorio si es aplicable | Si el conjunto de datos incluye varios archivos, indique la estructura de directorios y las relaciones entre los archivos] SPECIFIC INFORMATION FOR TABULAR DATA ------------------------------------------- [Este apartado se debe repetir para cada archivo de datos que requiera la explicación de variables (habitualmente datos tabulados). Se describirán todas las variables, incluyendo las unidades de medida.] 1. Name file: [Opcional | Nombre del archivo] 2. Number of rows and columns: [Opcional |Número de filas y columnas] 3. Variables list: [Opcional | Repita la estructura para cada variable.] Variable name: Description: Units of measure or value labels | Unidades de medida o etiquetas de valor: 4. Codes or symbols for missing data: [Opcional | Repita la estructura para cada código o símbolo que faltan] Code or symbol: Definition: 5. Special formats or abbreviations used: [Opcional |Formatos especiales o abreviaturas utilizadas] MORE INFORMATION -------------- [Include any other information about the dataset that is not reflected in this template and that you consider relevant. Incluya cualquier otra información sobre el conjunto de datos que no haya quedado reflejada en esta plantilla y que considere relevante]