Este archivo ha sido creado el 29-11-2023 por María Puerto GENERAL INFORMATION ------------------ 1. Dataset title: Genotoxicity evaluation of graphene in vitro [dataset] 2. Authorship: Name:Óscar Cebadero-Domínguez Institution:Universidad de Sevilla Email:ocebadero@us.es ORCID: 0000-0001-9481-562X Name:Concepción Medrano-Padial Institution:Universidad de Sevilla Email:cmpadial@us.es ORCID: 0000-0001-6358-3694 Name:María Puerto Institution:Universidad de Sevilla Email:mariapuerto@us.es ORCID: 0000-0003-3476-7456 Name:Soraya Sánchez Ballester Institution:ITENE Email:soraya.sanchez@itene.com ORCID: 0000-0002-9833-8776. Name:Ana María Cameán Institution:Universidad de Sevilla Email:camean@us.es ORCID: 0000-0003-1524-748X Name:Ángeles Jos Gallego Institution:Universidad de Sevilla Email:angelesjos@us.es ORCID: 0000-0001-7546-3294 DESCRIPTION ---------- 1. Dataset language:English 2. Abstract: The interest of graphene materials has increased markedly in the recent years for their promising applications in many fields as food packing. In this sense, a battery of in vitro test is required before its use as a food contact material. Then, the aim of this study was to assess the potential mutagenicity and genotoxicity of graphene oxide (GO) and reduced-graphene oxide (rGO) following the recommendations of the European Food Safety Authority (EFSA). Thus, the mouse lymphoma assay (MLA) and the micronucleus test (MN) were performed in L5178YTk ± cells, and the Caco-2 cells were used for the standard and modified comet assays. The results indicated that GO (0–250 µg/mL) was not mutagenic in the MLA. However, rGO revealed mutagenic activity from 250 µg/mL and 125 µg/mL after 4h and 24h of exposure, respectively. In the MN test, negative results were obtained for both compounds at the concentrations assayed (0–250 µg/mL) for GO/rGO. Moreover, no DNA strand breaks, or oxidative DNA damage were detected in Caco-2 cells exposed to GO (0–250 µg/mL) and rGO (0–176.3 µg/mL for 24h and 0–166.5 µg/mL for 48h). Dataset contains the numerical values of the results obtained MLA and comet assay, and MN test. 3. Keywords: graphene oxide, reduced-graphene oxide and genotoxicity 4. Date of data collection: 01-09-2021 6. Grant information: Grant Agency: Fondo Europeo de Desarrollo Regional (FEDER) y Consejería de Economía, Conocimiento, Empresas y Universidad de la Junta de Andalucía Grant Number: US-1259106 Grant Agency: PAIDI 2020/ FEDER, Consejería de Economía, Conocimiento, Empresas y Universidad de la Junta de Andalucía Grant Number: P18-RT-1993 ACCESS INFORMATION ------------------------ 1. Creative Commons License of the dataset:CC-BY 2. Dataset DOI: https://doi.org/10.12795/11441/152713 3. Related publication:Cebadero-Dominguez, Ó., Medrano-Padial, C., Puerto, M., Sánchez-Ballester, S., Cameán, A. M., & Jos, Á. (2023). Genotoxicity evaluation of graphene derivatives by a battery of in vitro assays. Chemico-biological interactions, 372, 110367. https://doi.org/10.1016/j.cbi.2023.110367 VERSIONING AND PROVENANCE --------------- 1. Last modification date: 29-06-2022 2. Were data derived from another source?: No METHODOLOGICAL INFORMATION ----------------------- We have study the potential mutagenicity and genotoxicity of graphene oxide (GO) and reduced-graphene oxide (rGO) following the recommendations of the European Food Safety Authority (EFSA). Thus, the mouse lymphoma assay and the micronucleus test were performed in L5178YTk ± cells, and the Caco-2 cells were used for the standard and modified comet assays. 1. Description of the methods used to collect and generate the data: The MLA was carried out according to OECD Guideline 490(2026) and Puerto et al. (2018). In vitro micronucleus assay was performed according to the OECD Guideline 487 and EFSA recommendations (2021). Standard alkaline comet assay was carried out to detect DNA strand breaks as previously described by Azqueta and Collins (2013)and Medrano-Padial et al. (2021) with modifications. References: Organization For Economic Co-operation and Development, OECD, Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene, vol. 490, OECD, Paris, France, 2016, https://doi.org/10.1787/9789264264908-en. No. 490). M. Puerto, A.I. Prieto, S. Maisanaba, D. Gutierrez-Praena, P. Mellado-García, A. Jos, A.M. Camean, Mutagenic and genotoxic potential of pure Cylindrospermopsin by a battery of in vitro tests, Food Chem. Toxicol. 121 (2018) 413–422, https://doi.org/10.1016/j.fct.2018.09.013 Mammalian Cell Micronucleus Test, OECD Guidelines for the Testing of Chemicals, vol. 4, OECD, Paris, France, 2016, https://doi.org/10.1787/9789264264861-en, 487 (No. 487) EFSA Scientific Committee, S. More, V. Bampidis, D. Benford, C. Bragard, T. Halldorsson, A. Hern´andez-Jerez, S. Hougaard Bennekou, K. Koutsoumanis, C. Lambre, H. Machera, K. Naegeli, S. Nielsen, J. Schlatter, D. Schrenk, V. Silano, D. Turck, M. Younes, J. Castenmiller, Q. Chaudhry, F. Cubadda, R. Franz, D. Gott, J. Mast, A. Mortensen, A.G. Oomen, S. Weigel, E. Barthelemy, A. Rincon, J. Tarazona, R. Schoonjans, Guidance on risk assessment of nanomaterials to be applied in the food and feed chain: human and animal health, EFSA J. 19 (8) (2021) 6768, https://doi.org/10.2903/j.efsa.2021.6768. AR A. Azqueta, A.R. Collins, The essential comet assay: a comprehensive guide to measuring DNA damage and repair, Arch. Toxicol. 87 (2013) 949–968, https://doi. org/10.1007/s00204-013-1070-0 C. Medrano-Padial, A.I. Prieto, M. Puerto, S. Pichardo, In vitro assessment of the mutagenic and genotoxic potential of a pure stilbene extract, Food Chem. Toxicol. 150 (2021), 112065, https://doi.org/10.1016/j.fct.2021.112065. 3. Software or instruments needed to interpret the data: Graph-Pad Prisma 9 version 9.0.0 software (Madrid, Spain), IBM SPSS Statistics (Madrid, Spain) and Comet Assay IV, Perceptive Instruments, UK. 4. Information about instruments, calibration and standards:The titration of FPG and Endo III was carried aut. FILE OVERVIEW ---------------------- 2. File list: File name:Cebadero et al., 2023 Chemic-Biol Interact 372 110367 [dataset] Description: The dataset is a excel file with 6 sheets with the raw data, medians, means and SD of the GO-MLA assay 4-24h, rGO-MLA assay 4-24h, GO-MN, rGO-Mn, GO-Comet assay-enz, rGO-Comet assay-enz 4. File format:Excel