Este archivo ha sido creado el 06.01.2025 por Matilde Durán Lobato GENERAL INFORMATION ------------------ 1. Dataset title: Dataset from Enhanced Cellular Uptake and Biodistribution of a Synthetic Cannabinoid Loaded in Surface-Modified PLGA Nanoparticles. Journal of Biomedical Nanotechnology, 10(1), 1-12 (2014) (v.0) 2. Authorship: Matilde Durán-Lobato Universidad de Sevilla Email: mduran@us.es ORCID: 0000-0001-5534-6955 Inmaculada Muñoz-Rubio Universidad de Sevilla Email: imunozrubio@us.es ORCID: N/A Mª Ángeles Holgado Universidad de Sevilla Email: holgado@us.es ORCID: 0000-0002-3851-3405 Josefa Álvarez-Fuentes Universidad de Sevilla Email: ffjalvarez@us.es ORCID: 0000-0002-3525-8488 Mercedes Fernández-Arévalo Universidad de Sevilla Email: mfarevalo@us.es ORCID: 0000-0002-4283-3356 Lucía Martín-Banderas Universidad de Sevilla Email: luciamartin@us.es ORCID: 0000-0003-1447-6125 DESCRIPTION ---------- 1. Dataset language: English 2. Abstract: This dataset contains experimental data on the characterization and evaluation of CB13-loaded, PLGA nanoparticles surface-modified with chitosan, lecithin, Eudragit®, and vitamin E. It includes further detailed drug encapsulation efficiency and loading efficiency, in vitro cellular viability and uptake, ex vivo mucoadhesion studies, and in vivo biodistribution assays in mice. 3. Keywords: Cannabinoid agonist, PLGA, nanoparticles, surface-modified, oral absorption enhancers Agonista cannabinoide, nanopartículas de PLGA, modificación de superficie, facilitadores de la absorción oral 4. Date of data collection: 01.10.2012 – 01.02.20114 5. Publication Date: 23-04-2014 6. Grant information: Grant Agency: Junta de Andalucía Grant Number: P09-CTS5029 Grant Agency: IV Plan Propio de Investigación, Universidad de Sevilla Grant Number: N/A (PIF Scholarship and Contrato de Acceso) 7. Geographical location/s of data collection: Facultad de Farmacia. Prof García González, 2, 41012 Sevilla, Andalucia, España Campus Reina Mercedes Coordinates: 37°21′39″N 5°59′18″O / 37.36094167, -5.98836111 ACCESS INFORMATION ------------------------ 1. Creative Commons License of the dataset: CC_BY 2. Dataset DOI: https://doi.org/10.12795/11441/166323 3. Related publication: Durán-Lobato, M., Muñoz-Rubio, I., Holgado, M. Á., Álvarez-Fuentes, J., Fernández-Arévalo, M., & Martín-Banderas, L. (2014). Enhanced Cellular Uptake and Biodistribution of a Synthetic Cannabinoid Loaded in Surface-Modified PLGA Nanoparticles. Journal of Biomedical Nanotechnology, 10(1), 1-12. DOI: 10.1166/jbn.2014.1806 4. Link to related datasets: VERSIONING AND PROVENANCE --------------- 1. Last modification date: 03-01-2025 2. Were data derived from another source?: NO 3. Additional related data not included in this dataset: METHODOLOGICAL INFORMATION ----------------------- 1. Description of the methods used to collect and generate the data: The dataset includes experimental data from the production and characterization of CB13-loaded PLGA nanoparticles (NPs) modified with surface agents such as chitosan, lecithin, Eudragit® RS, and vitamin E. NPs were prepared using the nanoprecipitation method. Particle size, polydispersity index (PDI), and zeta potential were measured using a Zetasizer Nano ZS (Malvern Instruments, UK). Entrapment efficiency and drug loading were quantified by reverse-phase high-performance liquid chromatography (RP-HPLC) on a Hitachi LaChrom® HPLC system. In vitro mucoadhesion studies were performed by incubating NPs with mucin to evaluate their binding efficiency. Cellular uptake and viability assays were conducted using Caco-2 cells. Cell viability was assessed via MTT assays, while flow cytometry and confocal microscopy were employed to evaluate NP internalization. In vivo biodistribution studies were carried out in mice, analyzing fluorescence signals from tissue homogenates after oral administration of fluorescently labeled NPs. 2. Data processing methods: Data were processed using SPSS for statistical analysis, including T-tests and ANOVA. Fluorescence intensity measurements were normalized, and HPLC was employed for drug quantification. MTT assay results were analyzed to determine cell viability, and mucoadhesion efficiency was calculated based on fluorescence intensity differences. 3. Software or instruments needed to interpret the data: N/A 4. Information about instruments, calibration and standards: Dynamic Light Scattering (DLS): Zetasizer Nano ZS (Malvern Instruments, UK), calibrated with polystyrene latex standards. HPLC System: Hitachi LaChrom® HPLC equipped with a Waters Spherisorb ODS2 column, calibrated with CB13 standards. Fluorescence Spectrophotometer: Synergy HT (BioTek Instruments, USA), calibrated with fluorescein standards for tissue fluorescence analysis. Flow Cytometer: Cytomics FC 500 MPL (Beckman Coulter, USA), calibrated with fluorescent bead standards. Confocal Laser Scanning Microscope (CLSM): Zeiss LSM 410 for qualitative cellular uptake studies. Tissue Homogenizer: Polytron® (Kinematica AG, Switzerland) for biodistribution studies. 5. Environmental or experimental conditions: N/A 6. Quality-assurance procedures performed on the data: [Opcional] FILE OVERVIEW ---------------------- 1. Explain the file naming conversion, si es aplicable: A single Excel file with each sheet containing: -Figure 3: Data corresponding to manuscript Figure 3 on entrapment efficiency and drug loading for each of the formulations assayed. -Figure 4: Data corresponding to manuscript Figure 4 on the drug release profile of the different formulations assayed. -Figure 6: Data corresponding to manuscript Figure 6 showing the % of mucoadhesion of the assayed formulations in different regions of the intestinal tract analyzed ex vivo. -Figure 7: Data corresponding to manuscript Figure 7 showing the effect of free CB13, blank PLGA NPs and loaded-PLGA NPs, plain and surface-modified on Caco-2 cells viability after an incubation period of 24h and 4 days. -Figure 8: Data corresponding to manuscript Figure 8 describing the uptake rate of plain and surface-modified NPs in Caco-2 cells -Figure 10: Data corresponding to manuscript Figure 10 showing tissue distribution represented as a measure of the relative percentage of particles detected related to the total particles. 2. File list: File name: Dataset from Enhanced Cellular Uptake and Biodistribution of a Synthetic Cannabinoid Loaded in Surface-Modified PLGA Nanoparticles Description: A single Excel file with each sheet containing: -Figure 3: Data corresponding to manuscript Figure 3 on entrapment efficiency and drug loading for each of the formulations assayed. -Figure 4: Data corresponding to manuscript Figure 4 on the drug release profile of the different formulations assayed. -Figure 6: Data corresponding to manuscript Figure 6 showing the % of mucoadhesion of the assayed formulations in different regions of the intestinal tract analyzed ex vivo. -Figure 7: Data corresponding to manuscript Figure 7 showing the effect of free CB13, blank PLGA NPs and loaded-PLGA NPs, plain and surface-modified on Caco-2 cells viability after an incubation period of 24h and 4 days. -Figure 8: Data corresponding to manuscript Figure 8 describing the uptake rate of plain and surface-modified NPs in Caco-2 cells -Figure 10: Data corresponding to manuscript Figure 10 showing tissue distribution represented as a measure of the relative percentage of particles detected related to the total particles. 3. Relationship between files: 4. File format: Excel 5. If the dataset includes multiple files, specify the directory structure and relationships between the files: