Este archivo ha sido creado el 2025-01-06 GENERAL INFORMATION ------------------ 1. Dataset title: Dataset from Ophthalmic wild olive (ACEBUCHE) oil nanoemulsions exert oculoprotective effects against oxidative stress induced by arterial hypertension. International Journal of Pharmaceutics, 2024, vol. 649, p. 123602. (v.0) 2. Authorship: Name: Álvaro Santana Garrido Institution: Universidad de Sevilla Email: asgarrido@us.es ORCID: 0000-0002-9469-3850 Name: Matilde Durán-Lobato Institution: Universidad de Sevilla Email: mduran@us.es RCID: 0000-0002-8646-7159 Name: Alfonso Mate Barrero Institution: Universidad de Sevilla Email: mate@us.es ORCID: 0000-0002-2719-8825 Name: Lucía Martín Banderas Institution: Universidad de Sevilla Email: luciamartin@us.es ORCID: 0000-0003-1447-6125 Name: Carmen María Vázquez Cueto Institution: Universidad de Sevilla Email: vazquez@us.es ORCID: 0000-0002-2999-3582 DESCRIPTION ---------- 1. Dataset language: English 2. Abstract: Dataset of the publication ‘Ophthalmic wild olive (acebuche) oil nanoemulsions exert oculoprotective effects against oxidative stress induced by arterial hypertension'. The work was primarily funded by the state project PID2019-109002RB-I00. The data present the development of a formulation based on acebuchina (ACE) oil nanoemulsions and its beneficial properties on the cornea and retina of animals with arterial hypertension induced by L-NAME. The data generated were collected between 2021 and 2023. 3. Keywords: Acebuche oil; Cornea; L-NAME hypertension; Nanoemulsion; Oxidative stress; Retina Acebuche; Córnea; Estrés oxidativo; Hipertensión arterial; Nanoemulsiones; Retina 4. Date of data collection: 2021-2023 5. Publication Date: 05-01-2024 6. Grant information: Grant Agency: Ministerio de Ciencia, Innovación y Universidades Grant Number: PID2019-109002RB-I00; 2020/275 Grant Agency: Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía Grant Number: 2020/275; 2021/188; CTS-584 7. Geographical location/s of data collection: Facultad de Farmacia. Prof García González, 2, 41012 Sevilla, Andalucia, España Campus Reina Mercedes Coordinates: 37°21′39″N 5°59′18″O / 37.36094167, -5.98836111 ACCESS INFORMATION ------------------------ 1. Creative Commons License of the dataset: CC_BY 2. Dataset DOI: https://doi.org/10.12795/11441/166300 3. Related publication: Santana-Garrido Á, Durán-Lobato M, Mate A, Martín-Banderas L, Vázquez CM. Ophthalmic wild olive (ACEBUCHE) oil nanoemulsions exert oculoprotective effects against oxidative stress induced by arterial hypertension. Int J Pharm. 2024 Jan 5;649:123602. doi: 10.1016/j.ijpharm.2023.123602. Epub 2023 Nov 13. PMID: 37967686. 4. Link to related datasets: VERSIONING AND PROVENANCE --------------- 1. Last modification date: 2. Were data derived from another source?: NO 3. Additional related data not included in this dataset: METHODOLOGICAL INFORMATION ----------------------- 1. Description of the methods used to collect and generate the data: The dataset includes data from the characterization of acebuche oil nanoemulsions, including physicochemical parameters (e.g., size, PDI, ZP, osmolality and pH), rheology and colloidal stability. In vivo experiments were conducted in arterial hypertensive mice, analyzing corneal and retinal layer thickness, reactive oxygen species and NADPH oxidase activity. All measurements were performed at least in triplicates and expressed as mean ± standard deviation. 2. Data processing methods: NADPH oxidase activity was measured by lucigenin-enhanced chemiluminescence using BERTHOLD Junior LB 9509. CFX96 real-time PCR system (Bio-Rad, Madrid, Spain) was used mRNA NOX expression. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a housekeeping gene to quantify the relative changes in mRNA expression following the 2-2ΔΔCt method. Paraffin-embedded eye sections were used to estimate in situ ROS production by dihydroethidium (DHE) staining (MedChemExpress, Madrid, Spain, Cat. No. HY-D0079) in the cornea and retina. An Olympus DP73 fluorescence microscope (Tokyo, Japan) and Image J-NIH freeware (v. 2.0.0) were used to measure staining intensities. All images were taken using the same exposure time and magnification. Analysis were done in the same corneal/retinal area. Results were expressed as relative to the Control group. Statistical analysis was performed using GraphPad Prism. 3. Software or instruments needed to interpret the data: N/A 4. Information about instruments, calibration and standards: Statistical and Data Analysis Software: All statistical calculations and graphical representations were performed using GraphPad Prism. Fluorescence data were normalized and processed for quantification. 5. Environmental or experimental conditions: N/A 6. Quality-assurance procedures performed on the data: FILE OVERVIEW ---------------------- 1. Explain the file naming conversion: A single Excel file containing ten sheets, each named after the table or figure in the manuscript that corresponds to the data it contains: -Table 2: data corresponding to manuscript Table 2 on the physicochemical characterization of the nanoemulsions (NEs) -Figure 3: data corresponding to manuscript Figure 3 displaying the rheological evaluation of the NEs -Figure 5A-B: data corresponding to manuscript Figure 5A-B displaying the corneal layer thickness measurements. -Figure 5C-D: data corresponding to manuscript Figure 5A-B displaying the retinal layer thickness measurements. -Figure 6: data corresponding to manuscript Figure 6 regarding reactive oxygen species (ROS) measured by DHE staining in the corneal layers. -Figure 7: data corresponding to manuscript Figure 7 regarding ROS measured by DHE staining in the retinal layers. -Figure 8A-B: data corresponding to manuscript Figure 8A-B regarding NADPH oxidase activity and ROS sources in cornea homogenates. -Figure 8C: data corresponding to manuscript Figure 8C displaying the corneal NOX isoform mRNA expression. -Figure 8D-E: data corresponding to manuscript Figure 8D-E regarding NADPH oxidase activity and ROS sources in retina homogenates. -Figure 8F: data corresponding to manuscript Figure 8F displaying the retinal NOX isoform mRNA expression. 2. File list: File name: Description: A single Excel file with each sheet collecting the data corresponding to a figure or table: -Table 2: data corresponding to manuscript Table 2 on the physicochemical characterization of the nanoemulsions (NEs) -Figure 3: data corresponding to manuscript Figure 3 displaying the rheological evaluation of the NEs -Figure 5A-B: data corresponding to manuscript Figure 5A-B displaying the corneal layer thickness measurements. -Figure 5C-D: data corresponding to manuscript Figure 5A-B displaying the retinal layer thickness measurements. -Figure 6: data corresponding to manuscript Figure 6 regarding reactive oxygen species (ROS) measured by DHE staining in the corneal layers. -Figure 7: data corresponding to manuscript Figure 7 regarding ROS measured by DHE staining in the retinal layers. -Figure 8A-B: data corresponding to manuscript Figure 8A-B regarding NADPH oxidase activity and ROS sources in cornea homogenates. -Figure 8C: data corresponding to manuscript Figure 8C displaying the corneal NOX isoform mRNA expression. -Figure 8D-E: data corresponding to manuscript Figure 8D-E regarding NADPH oxidase activity and ROS sources in retina homogenates. -Figure 8F: data corresponding to manuscript Figure 8F displaying the retinal NOX isoform mRNA expression. 3. Relationship between files: 4. File format: Excel 5. If the dataset includes multiple files, specify the directory structure and relationships between the files: