Este archivo ha sido creado el 20-07-2024 por Eduardo Ponce-España GENERAL INFORMATION ------------------ 1. Dataset title: Anti-obesogenic effects of vegetable-derived protein hydrolysate [Dataset] 2. Authorship: Name: Eduardo Ponce-España Institution: Universidad de Sevilla Email: eponce-ibis@us.es ORCID: 0000-0001-6197-0041 Name: Ivan Cruz-Chamorro Institution: Universidad de Sevilla Email: icruz-ibis@us.es ORCID: 0000-0002-6547-8078 Name: Guillermo Santos-Sánchez Institution: Universidad de Sevilla Email: gsantos-ibis@us.es ORCID: 0000-0001-5790-2302 Name: Ana Isabel Álvarez-López Institution: Instituto de Biomedicina de Sevilla Email: aialvarez-ibis@us.es ORCID: 0000-0002-9408-0472 Name: José María Fernández-Santos Institution: Universidad de Sevilla Email: jmsantos@us.es ORCID: 0000-0003-4541-1033 Name: Justo Pedroche Institution: Instituto de la Grasa Email: j.pedroche@csic.es ORCID: 0000-0002-2562-3779 Name: María Carmen Millán-Linares Institution: Instituto de la Grasa Email: mcmillan@ig.csic.es ORCID: 0000-0002-5661-8366 Name: Ignacio Bejarano Institution: Universidad de Sevilla Email: ibejarano@us.es ORCID: 0000-0003-3727-3873 Name: Patricia Judith Lardone Institution: Universidad de Sevilla Email: plardone@us.es ORCID: 0000-0003-1793-3985 Name: Antonio Carrillo-Vico Institution: Universidad de Sevilla Email: vico@us.es ORCID: 0000-0002-8516-0999 DESCRIPTION ---------- 1. Dataset language: Inglés 2. Abstract: Data was acquired for the study published in the article titled: Anti-obesogenic effect of lupin-derived protein hydrolysate through modulation of adiposopathy, insulin resistance and gut dysbiosis in a diet-induced obese mouse. This study examines the effects of a lupin protein hydrolysate (LPH) on obesity, adipose tissue dysfunction and gut dysbiosis in obese mice. LPH was characterized and protein concentration, ashes, moisture, fiber, fats, soluble sugars and phenol content were measured as described. Amino acid composition was determined using standard amino acid mix solution. The molecular weight profile was obtained by molecular exclusion chromatography. Eight-week-old male C57BL6/N mice were housed under standard conditions and mice were randomly assigned to three groups: mice fed a standard diet (SD), a high-fat diet (HFD) or an HFD and treated intragastrically with LPH (HFD+LPH) at a dose of 100mg/kg five days a week for 12 weeks. The SD and HFD groups were treated with vehicle under the same conditions as the HFD+LPH group. Food consumption and individual body weight were measured weekly. Animals were sacrificed with an intraperitoneal injection of sodium thiopental and blood was collected by cardiac puncture. Epidydimal adipose tissue (EpiWAT) was dissected, snap-frozen and stored at -80ºC until use or fixed in a 4% paraformaldehyde (PFA) solution. Stool was collected prior to euthanasia and stored at -80ºC. Serum biochemical profile was measured by chemoluminiscence in the Cobas Integra 400 (Roche Diagnostics, Indianapolis, IN, USA) at the Estación Biológica de Doñana (EBD-CSIC, Seville, Spain). Serum concentrations of leptin, adiponectin and insulin were quantified using enzyme-linked immunosorbent assays (ELISA) kits. Adipose tissue samples fixed in 4% PFA were dehydrated, embedded in paraffin blocks and sliced 4μm thick, stained with hematoxylin-eosin and cover mounted for posterior observation. Photomicrographs were obtained using Leica THUNDER microscope and Leica Application Suite X software. Adipocyte morphology and number of crown-like structures was blinded analyzed using ImageJ v1.53h public software. High throughput 16S rRNA gene amplicon analysis was performed using total DNA extracted from stool. 16S rRNA V3–V4 region was sequenced using MiSeq protocol and instruments. The raw sequences were processed using mothur. 3. Keywords: hydrolysate; obesity; adipose tissue; microbiota 4. Date of data collection (fecha única o rango de fechas): 02-12-2020 - 31-03-2024 5. Publication Date: 01-08-2024 6. Grant information: Grant Agency: Andalusian Government Ministry of Health Grant Number: PEMP-0085-2020 Grant Agency: Andalusian Government Grant Number: CTS160 Grant Agency: Ministerio de Ciencia e Innovación Grant Number: NutriCropRED2022-134382-T Grant Agency: VI Program of Inner Initiative for Research and Transfer of the University of Seville Grant Number: VI PPIT-US Grant Agency: Ministerio de Educación, Cultura y Deporte Grant Number: FPU16/02339 Grant Agency: Andalusian Government Ministry of Economy, Knowledge, Business, and University Grant Number: DOC_00587/2020 7. Geographical location/s of data collection: 37.36461597772077, -5.9786894560670305, Instituto de Biomedicina de Sevilla, Seville, Andalusia, Spain ACCESS INFORMATION ------------------------ 1. Creative Commons License of the dataset: CC BY-NC-ND 2. Dataset DOI: https://doi.org/10.12795/11441/161833 3. Related publication: Ponce-España, E., Cruz-Chamorro, I., Santos-Sánchez, G., Álvarez-López, A. I., Fernández-Santos, J. M., Pedroche, J., Millán-Linares, M. C., Bejarano, I., Lardone, P. J., & Carrillo-Vico, A. (2024). Anti-obesogenic effect of lupin-derived protein hydrolysate through modulation of adiposopathy, insulin resistance and gut dysbiosis in a diet-induced obese mouse. Biomedicine & Pharmacotherapy, 178, 117198. https://doi.org/10.1016/j.biopha.2024.117198 4. Link to related datasets: DOI/URL: VERSIONING AND PROVENANCE --------------- 1. Last modification date: 31-03-2024 2. Were data derived from another source?: No METHODOLOGICAL INFORMATION ----------------------- 1. Description of the methods used to collect and generate the data: Protein concentration, ashes, moisture, fiber, fats, soluble sugars and phenol content of LPH were measured as described elsewhere (10.1016/j.foodres.2019.108585). Amino acid composition was determined using the method developed by Alaiz et al. (10.1016/0021-9673(92)80236-N) using standard amino acid mix solution from Sigma (St. Louis, MO, EEUU). The molecular weight profile was obtained by molecular exclusion chromatography using a Superose 12HR 10/30 column (exclusion limit 2 × 106 Da and optimal separation range 1000-3 × 105 Da). Serum concentration of cholesterol, HDL, LDL, triglycerides and glucose were measured by chemoluminiscence in the Cobas Integra 400 (Roche Diagnostics, Indianapolis, IN, USA) at the Estación Biológica de Doñana (EBD-CSIC, Seville, Spain). The serum concentrations of leptin, adiponectin and insulin were quantified using enzyme-linked immunosorbent assays (ELISA) kits from Biovendor (RD291001200R; RD293023100R) and Mercodia (10-1247-01), respectively, using the manufacturer’s instructions. Photomicrographs were obtained using Leica THUNDER microscope (Leica) and Leica Application Suite X software (Leica Microsystems). Adipocyte morphology (size and number) and number of crown-like structures (CLS) was blinded analyzed using ImageJ v1.53h public software (NIH, Bethesda, MD, USA). Amplicon-sequencing targeting of the 16S rRNA gene was performed at the Genomics Core Facility of the IBiS. The amplicon libraries were prepared using the primer of the V3–V4 region according to the 16S metagenomic sequencing library preparation protocol (Part # 15044223 Rev. B). The libraries were pooled at 8 pM concentrations before loading onto the MiSeq (Illumina, San Diego, CA, USA) using a Reagent Kit v3 (300 cycles) in paired-end mode, with 25% PhiX added for clear cluster separation. The raw sequences were processed using mothur (10.1128/AEM.01541-09). The reads were trimmed based on quality scores and aligned with the SILVA 138.1 database (10.1093/nar/gks1219). Chimeric sequences were removed using the VSEARCH algorithm (10.7717/peerj.2584) and reads classified as “eukaryote”, “mitochondria”, “chloroplast” or “unknown” were removed. Operational taxonomic units (OTUs) were clustered at 97% of sequence similarity and the final taxonomic identification was assigned with the SILVA 138.1 database. Ecological index (Shannon and Sobs), and dissimilarity between communities (Jaccard index) were calculated using mothur subroutines. Differential abundance analysis was performed using the Linear discriminant analysis Effect Size (LEfSe) algorithm (10.1186/gb-2011-12-6-r60) 2. Data processing methods: For discriminant analysis morphometrical parameters (body weight, adipose tissue weight) and serum lipids (cholesterol, triglycerides, LDL, HDL) were used to create canonical discriminant functions. Spearman's rank correlation coefficients were calculated to create a correlation matrix between the gut microbiota profile and obesity markers. Both analysis were performed using SPSS® Stat Software v26 (IBM Corporation, Armonk, NY, USA) Regarding adipocyte morphology (size and number) and of crown-like structures (CLS) frequency, microphotographs were blinded analyzed using ImageJ v1.53h public software (NIH, Bethesda, MD, USA) 3. Software or instruments needed to interpret the data: Graphs were designed using GraphPad Prism v8 software (GraphPad Software, Boston, MA, USA) 4. Information about instruments, calibration and standards: See "1. Description of the methods used to collect and generate the data" section FILE OVERVIEW ---------------------- 2. File list: [Obligatorio | Repita el esquema para cada archivo.] File name: hydrolysate_composition.xlsx Description: Macromolecular and aminoacidic composition (first sheet) and chromatogram information (second sheet) of LPH obtained from three independent measurements. File name: obesity_markers.xlsx Description: Experimental group and numerical data (columns) obtained from the measurement of parameters from animals (rows) used in the present study. 4. File format: Excel (.xlsx) 5. If the dataset includes multiple files, specify the directory structure and relationships between the files: Not related files SPECIFIC INFORMATION FOR TABULAR DATA 1 ------------------------------------------- 1. Name file: hydrolysate_composition.xlsx 2. Number of rows and columns: -1st sheet: 27 rows and 3 columns -2nd sheet: 963 rows and 4 columns 3. Variables list: - 1st sheet: Variable name: Quantity Description: Measured values of every compound Units of measure or value labels | Unidades de medida o etiquetas de valor: gram per 100 grams Variable name: Standard_deviation Description: Standard deviaton of the measured values of every copound Units of measure or value labels | Unidades de medida o etiquetas de valor: gram per 100 grams - 2nd sheet: Variable name: Eluted volume Description: Eluted volume through chromatography column Units of measure or value labels | Unidades de medida o etiquetas de valor: mililitres Variable name: 1st-2nd-3rd_replicate Description: Absorbance units measured in chromatography column Units of measure or value labels | Unidades de medida o etiquetas de valor: absorbance units SPECIFIC INFORMATION FOR TABULAR DATA 2 ------------------------------------------- 1. Name file: obesity_markers.xlsx 2. Number of rows and columns: 87 rows and 76 columns 3. Variables list: Variable name: Initial_weight (g) Description: Initial body weight Units of measure or value labels | Unidades de medida o etiquetas de valor: grams Variable name: Body_Weight_Week1 (g) Description: Body weight at week 1 Units of measure or value labels | Unidades de medida o etiquetas de valor: grams Variable name: Body_Weight_Week2 (g) Description: Body weight at week 2 Units of measure or value labels | Unidades de medida o etiquetas de valor: grams Variable name: Body_Weight_Week3 (g) Description: Body weight at week 3 Units of measure or value labels | Unidades de medida o etiquetas de valor: grams Variable name: Body_Weight_Week4 (g) Description: Body weight at week 4 Units of measure or value labels | Unidades de medida o etiquetas de valor: grams Variable name: Body_Weight_Week5 (g) Description: Body weight at week 5 Units of measure or value labels | Unidades de medida o etiquetas de valor: grams Variable name: Body_Weight_Week6 (g) Description: Body weight at week 6 Units of measure or value labels | Unidades de medida o etiquetas de valor: grams Variable name: Body_Weight_Week7 (g) Description: Body weight at week 7 Units of measure or value labels | Unidades de medida o etiquetas de valor: grams Variable name: Body_Weight_Week8 (g) Description: Body weight at week 8 Units of measure or value labels | Unidades de medida o etiquetas de valor: grams Variable name: Body_Weight_Week9 (g) Description: Body weight at week 9 Units of measure or value labels | Unidades de medida o etiquetas de valor: grams Variable name: Body_Weight_Week10 (g) Description: Body weight at week 10 Units of measure or value labels | Unidades de medida o etiquetas de valor: grams Variable name: Body_Weight_Week11 (g) Description: Body weight at week 11 Units of measure or value labels | Unidades de medida o etiquetas de valor: grams Variable name: Body_Weight_Week12 (g) Description: Body weight at week 12 Units of measure or value labels | Unidades de medida o etiquetas de valor: grams Variable name: EpiWAT_weight (g) Description: Weight of adipose tissue Units of measure or value labels | Unidades de medida o etiquetas de valor: grams Variable name: Cholesterol (mg/dL) Description: Serum concetration of cholesterol Units of measure or value labels | Unidades de medida o etiquetas de valor: miligram/decilitre Variable name: LDL (mmol/L) Description: Serum concetration of LDL Units of measure or value labels | Unidades de medida o etiquetas de valor: milimol/litre Variable name: HDL (mmol/L) Description: Serum concetration of HDL Units of measure or value labels | Unidades de medida o etiquetas de valor: milimol/litre Variable name: Triglycerides (mgdL) Description: Serum concetration of Tryglycerides Units of measure or value labels | Unidades de medida o etiquetas de valor: miligram/decilitre Variable name: Glucose (mg/dL) Description: Serum concetration of Glucose Units of measure or value labels | Unidades de medida o etiquetas de valor: miligram/decilitre Variable name: Insulin (μg/L) Description: Serum concetration of insulin Units of measure or value labels | Unidades de medida o etiquetas de valor: microgram/litre Variable name: Leptin (μg/L) Description: Serum concetration of leptin Units of measure or value labels | Unidades de medida o etiquetas de valor: microgram/litre Variable name: Adiponectin (μg/L) Description: Serum concetration of Adiponectin Units of measure or value labels | Unidades de medida o etiquetas de valor: microgram/litre Variable name: Shannon_Index Description: Alpha diversity index Units of measure or value labels | Unidades de medida o etiquetas de valor: AU Variable name: Richness (sobs) Description: Richness of species observed Units of measure or value labels | Unidades de medida o etiquetas de valor: "species" observed Variable name: Bacteroidota (%seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Bacillota (%seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Pseudomonadota (%seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Actinomycetota (%seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Deferribacterota(%seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Cyanobacteriota (%seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Patescibacteria (%seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Thermodesulfobacterota (%seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Ruminococcus (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Prevotellaceae_UCG_001 (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Allobaculum (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Lachnospiraceae_UCG_001 (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Muribaculaceae_ge (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Lachnospiraceae_NK4A136_group (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Lachnospiraceae_UCG_006 (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Desulfovibrio (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Ruminococcaceae_unclassified (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Candidatus_Saccharimonas (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Oscillibacter (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Negativibacillus (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Parabacteroides (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Oscillospiraceae_unclassified (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Colidetribacter (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Lachnoclostridium (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Bacteroides (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Tyzzerella (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Turicibacter (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Ileibacterium (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Clostridium_sensu_stricto_1 (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Rikenellaceae_RC9_gut_group (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Alloprevotella (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Parasutterella (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Dubosiella (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Akkermansia (% seqs) Description: % of sequences corresponding to this taxa Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of sequences Variable name: Adipocity diameter (<30μm) Description: Frequence of adipocity with this diameter Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of total adipocytes Variable name: Adipocity diameter (30-40 μm) Description: Frequence of adipocity with this diameter Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of total adipocytes Variable name: Adipocity diameter (40-50 μm) Description: Frequence of adipocity with this diameter Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of total adipocytes Variable name: Adipocity diameter (50-60 μm) Description: Frequence of adipocity with this diameter Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of total adipocytes Variable name: Adipocity diameter (60-70 μm) Description: Frequence of adipocity with this diameter Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of total adipocytes Variable name: Adipocity diameter (70-80 μm) Description: Frequence of adipocity with this diameter Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of total adipocytes Variable name: Adipocity diameter (80-90 μm) Description: Frequence of adipocity with this diameter Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of total adipocytes Variable name: Adipocity diameter (90-100 μm) Description: Frequence of adipocity with this diameter Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of total adipocytes Variable name: Adipocity diameter (100-110 μm) Description: Frequence of adipocity with this diameter Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of total adipocytes Variable name: Adipocity diameter (110-120 μm) Description: Frequence of adipocity with this diameter Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of total adipocytes Variable name: Adipocity diameter (120-130 μm) Description: Frequence of adipocity with this diameter Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of total adipocytes Variable name: Adipocity diameter (130-140 μm) Description: Frequence of adipocity with this diameter Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of total adipocytes Variable name: Adipocity diameter (140-150 μm) Description: Frequence of adipocity with this diameter Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of total adipocytes Variable name: Adipocity diameter (150-160 μm) Description: Frequence of adipocity with this diameter Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of total adipocytes Variable name: Adipocity diameter (>160 μm) Description: Frequence of adipocity with this diameter Units of measure or value labels | Unidades de medida o etiquetas de valor: percentage of total adipocytes Variable name: Frequency of CLS (number/μm^2) Description: Frequence of crown-like structures per mm^2 Units of measure or value labels | Unidades de medida o etiquetas de valor: number of crown-like structures divided by squared milimeter of tissue