Artículo
Crosstalk between Virulence Loci: Regulation of Salmonella enterica Pathogenicity Island 1 (SPI-1) by Products of the std Fimbrial Operon
Autor/es | López Garrido, Javier
Casadesús Pursals, Josep |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2012 |
Fecha de depósito | 2018-12-13 |
Publicado en |
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Resumen | Invasion of intestinal epithelial cells is a critical step in
Salmonella
infection and requires the expression of genes located in
Salmonella
pathogenicity island 1 (SPI-1). A key factor for SPI-1 expression is DNA ... Invasion of intestinal epithelial cells is a critical step in Salmonella infection and requires the expression of genes located in Salmonella pathogenicity island 1 (SPI-1). A key factor for SPI-1 expression is DNA adenine (Dam) methylation, which activates synthesis of the SPI-1 transcriptional activator HilD. Dam-dependent regulation of hilD is postranscriptional (and therefore indirect), indicating the involvement of unknown cell functions under Dam methylation control. A genetic screen has identified the std fimbrial operon as the missing link between Dam methylation and SPI-1. We show that all genes in the std operon are part of a single transcriptional unit, and describe three previously uncharacterized ORFs (renamed stdD , stdE , and stdF ). We present evidence that two such loci ( stdE and stdF ) are involved in Dam-dependent control of Salmonella SPI- 1: in a Dam 2 background, deletion of stdE or stdF suppresses SPI-1 repression; in a Dam + background, constitutive expression of StdE and/or StdF represses SPI-1. Repression of SPI-1 by products of std operon explains the invasion defect of Salmonella Dam 2 mutants, which constitutively express the std operon. Dam-dependent repression of std in the ileum may be required to permit invasion, as indicated by two observations: constitutive expression of StdE and StdF reduces invasion of epithelial cells in vitro (1,000 fold) and attenuates Salmonella virulence in the mouse model ( . 60 fold). In turn, crosstalk between std and SPI-1 may play a role in intestinal infections by preventing expression of SPI-1 in the caecum, an intestinal compartment in which the std operon is known to be expressed. |
Agencias financiadoras | Ministerio de Ciencia e Innovación (MICIN). España |
Identificador del proyecto | BIO2010-15023
CSD2008-0001 |
Cita | López Garrido, J. y Casadesús Pursals, J. (2012). Crosstalk between Virulence Loci: Regulation of Salmonella enterica Pathogenicity Island 1 (SPI-1) by Products of the std Fimbrial Operon. PLOS One, 7 (1), e30499. |
Ficheros | Tamaño | Formato | Ver | Descripción |
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journal.pone.0030499.PDF | 905.8Kb | [PDF] | Ver/ | |