Artículo
A purL mutant of Sinorhizobium fredii HH103 is symbiotically defective and altered in its lipopolysaccharide
Autor/es | Buendía Clavería, Ana María
Moussaid, Ahmed Ollero Márquez, Francisco Javier Vinardell González, José María Torres Rueda, Antonio Ildefonso Moreno Onorato, Francisco Javier Gil Serrano, Antonio Miguel Rodríguez Carvajal, Miguel Ángel Tejero Mateo, María Pilar Ruiz Sainz, José Enrique |
Departamento | Universidad de Sevilla. Departamento de Biología Celular Universidad de Sevilla. Departamento de Química orgánica Universidad de Sevilla. Departamento de Microbiología |
Fecha de publicación | 2003 |
Fecha de depósito | 2016-04-22 |
Publicado en |
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Resumen | The pleiotropic phenotype of an auxotrophic purL mutant (SVQ295) of Sinorhizobium fredii HH103
has been investigated. SVQ295 forms colonies that are translucent, produce more slime and
absorb less Congo red than those ... The pleiotropic phenotype of an auxotrophic purL mutant (SVQ295) of Sinorhizobium fredii HH103 has been investigated. SVQ295 forms colonies that are translucent, produce more slime and absorb less Congo red than those of wild-type strain HH103. SVQ295 did not grow in minimal medium unless the culture was supplemented with thiamin and adenine or with thiamin and AICA-riboside (5-aminoimidazole-4-carboxamide 1-b-D-ribofuranoside), an intermediate of purine biosynthesis. Bacterial cultures supplemented with AICA-riboside or adenine reached the same culture density, although the doubling time of SVQ295 cultures containing AICA-riboside was clearly longer. S. fredii SVQ295 induced pseudonodules on Glycine max and failed to nodulate six different legumes. On Glycyrrhiza uralensis, however, nodules showing nitrogenase activity and containing infected plant cells were formed. SVQ295 showed auto-agglutination when grown in liquid TY medium and its lipopolysaccharide (LPS) electrophoretic profile differed from that of its parental strain HH103-1. In addition, four monoclonal antibodies that recognize the LPS of S. fredii HH103 failed to recognize the LPS produced by SVQ295. In contrast, 1H-NMR spectra of K-antigen capsular polysaccharides (KPS) produced by SVQ295 and the wild-type strain HH103 were similar. Co-inoculation of soybean plants with SVQ295 and SVQ116 (a nodA mutant derivative of HH103) produced nitrogen-fixing nodules that were only occupied by SVQ116. |
Cita | Buendía Clavería, A.M., Moussaid, A., Ollero Márquez, F.J., Vinardell González, J.M., Torres Rueda, A.I., Moreno Onorato, F.J.,...,Ruiz Sainz, J.E. (2003). A purL mutant of Sinorhizobium fredii HH103 is symbiotically defective and altered in its lipopolysaccharide. Microbiology, 149 (7), 1807-1818. |
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