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Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity

dc.creatorAlbi Rodríguez, Tomáses
dc.creatorOrtiz Marchena, M Isabeles
dc.creatorRuiz Pérez, Teresaes
dc.creatorRomero Rodríguez, José Maríaes
dc.creatorValverde Albacete, Federicoes
dc.date.accessioned2019-02-06T08:07:38Z
dc.date.available2019-02-06T08:07:38Z
dc.date.issued2014
dc.identifier.citationAlbi Rodríguez, T., Ortiz Marchena, M.I., Ruiz Pérez, T., Romero Rodríguez, J.M. y Valverde Albacete, F. (2014). Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity. Bio-protocol, 4 (23), 1-8.
dc.identifier.issn2331-8325es
dc.identifier.urihttps://hdl.handle.net/11441/82556
dc.description.abstractStarch constitutes the most important carbon reserve in plants and is composed of branched amylopectin and linear amylose. The latter is synthesized exclusively by the Granule-Bound Starch Synthase (GBSS, EC 2.4.1.21). Here we report a readily reproducible, specific and highly sensitive protocol, which includes the isolation of intact starch granules from Arabidopsis thaliana leaves and the subsequent determination of GBSS activity. We have applied this method to study GBSS activity in diurnal cycles in vegetative growth and during the photoperiodic transition to flowering in Arabidopsis (Tenorio et al., 2003; Ortiz-Marchena et al., 2014).es
dc.description.sponsorshipEspaña,MINECO CSD2007-00057, BIO2008-02292, and BIO2011-28847-C02-00es
dc.description.sponsorshipEspaña, Junta de Andalucía P06-CVI-01450 and P08-AGR-03582es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherBio-Protocoles
dc.relation.ispartofBio-protocol, 4 (23), 1-8.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectStarch granulees
dc.subjectStarch purificationes
dc.subjectGBSS activityes
dc.subjectGranule-bound starch synthasees
dc.titlePurification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activityes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.projectIDCSD2007-00057, BIO2008-02292, and BIO2011-28847-C02-00es
dc.relation.projectIDP06-CVI-01450 and P08-AGR-03582es
dc.relation.publisherversionhttp://dx.doi.org/10.21769/BioProtoc.1316es
dc.identifier.doi10.21769/BioProtoc.1316es
idus.format.extent8 p.es
dc.journaltitleBio-protocoles
dc.publication.volumen4es
dc.publication.issue23es
dc.publication.initialPage1es
dc.publication.endPage8es
dc.contributor.funderMinisterio de Economía y Competitividad (MINECO). España
dc.contributor.funderJunta de Andalucía

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