Artículo
Inhibition of Type 1 Fimbriae-mediated Escherichia Coli Adhesion and Biofilm Formation by Trimeric Cluster Thiomannosides Conjugated to Diamond Nanoparticles
Autor/es | Khanal, Manakamana
Larsonneur, Fanny Raks, Victoriia Barras, Alexandre Baumann, Jean-Sébastien Martin, Fernando Ariel Martin, Fernando Ariel Boukherroub, Rabah Ghigo, Jean Marc Ortiz Mellet, Carmen Zaitsev, Vladimir Garcia Fernandez, Jose M. Beloin, Christophe Siriwardena, Aloysius Szunerits, Sabine |
Departamento | Universidad de Sevilla. Departamento de Química orgánica |
Fecha de publicación | 2015 |
Fecha de depósito | 2022-11-11 |
Publicado en |
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Resumen | Recent advances in nanotechnology have seen the development of a number of microbiocidal and/or anti-adhesive nanoparticles displaying activity against biofilms. In this work, trimeric thiomannoside clusters conjugated to ... Recent advances in nanotechnology have seen the development of a number of microbiocidal and/or anti-adhesive nanoparticles displaying activity against biofilms. In this work, trimeric thiomannoside clusters conjugated to nanodiamond particles (ND) were targeted for investigation. NDs have attracted attention as a biocompatible nanomaterial and we were curious to see whether the high mannose glycotope density obtained upon grouping monosaccharide units in triads might lead to the corresponding ND-conjugates behaving as effective inhibitors of E. coli type 1 fimbriae-mediated adhesion as well as of biofilm formation. The required trimeric thiosugar clusters were obtained through a convenient thiol-ene "click" strategy and were subsequently conjugated to alkynyl-functionalized NDs using a Cu(I)-catalysed "click" reaction. We demonstrated that the tri-thiomannoside cluster-conjugated NDs (ND-Man3) show potent inhibition of type 1 fimbriae-mediated E. coli adhesion to yeast and T24 bladder cells as well as of biofilm formation. The biofilm disrupting effects demonstrated here have only rarely been reported in the past for analogues featuring such simple glycosidic motifs. Moreover, the finding that the tri-thiomannoside cluster (Man3N3) is itself a relatively efficient inhibitor, even when not conjugated to any ND edifice, suggests that alternative mono- or multivalent sugar-derived analogues might also be usefully explored for E. coli-mediated biofilm disrupting properties. |
Agencias financiadoras | French National Research Agency (ANR) Fondation pour la Recherche Medicale (FRM) Gobierno de España Junta de Andalucía European Union (UE) European Cooperation in Science and Technology (COST) |
Identificador del proyecto | 3905-1
ANR-10-LABX-62-IBEID DEQ20140329508 SAF2013-44021-R CTQ2010-15848 269099 CM1102 |
Cita | Khanal, M., Larsonneur, F., Raks, V., Barras, A., Baumann, J., Martin, F.A.,...,Szunerits, S. (2015). Inhibition of Type 1 Fimbriae-mediated Escherichia Coli Adhesion and Biofilm Formation by Trimeric Cluster Thiomannosides Conjugated to Diamond Nanoparticles. Nanoscale, 7 (6), 2325-2335. https://doi.org/10.1039/c4nr05906a. |
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