Artículo
Plant responses to fungal volatiles involve global posttranslational thiol redox proteome changes that affect photosynthesis
Autor/es | Ameztoy, Kinia
Baslam, Marouane Sánchez López, Ángela María Muñoz, Francisco José Bahaji, Abdellatif Almagro, Goizeder García Gómez, Pablo Baroja Fernández, Edurne De Diego, Nuria Humplík, Jan F. Cejudo Fernández, Francisco Javier Pozueta Romero, Javier |
Departamento | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Fecha de publicación | 2019 |
Fecha de depósito | 2022-02-21 |
Publicado en |
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Resumen | Microorganisms produce volatile compounds (VCs) that promote plant growth and photosynthesis through complex mechanisms involving cytokinin (CK) and abscisic acid (ABA). We hypothesized that plants' responses to microbial ... Microorganisms produce volatile compounds (VCs) that promote plant growth and photosynthesis through complex mechanisms involving cytokinin (CK) and abscisic acid (ABA). We hypothesized that plants' responses to microbial VCs involve posttranslational modifications of the thiol redox proteome through action of plastidial NADPH-dependent thioredoxin reductase C (NTRC), which regulates chloroplast redox status via its functional relationship with 2-Cys peroxiredoxins. To test this hypothesis, we analysed developmental, metabolic, hormonal, genetic, and redox proteomic responses of wild-type (WT) plants and a NTRC knockout mutant (ntrc) to VCs emitted by the phytopathogen Alternaria alternata. Fungal VC-promoted growth, changes in root architecture, shifts in expression of VC-responsive CK- and ABA-regulated genes, and increases in photosynthetic capacity were substantially weaker in ntrc plants than in WT plants. As in WT plants, fungal VCs strongly promoted growth, chlorophyll accumulation, and photosynthesis in ntrc–Δ2cp plants with reduced 2-Cys peroxiredoxin expression. OxiTRAQ-based quantitative and site-specific redox proteomic analyses revealed that VCs promote global reduction of the thiol redox proteome (especially of photosynthesis-related proteins) of WT leaves but its oxidation in ntrc leaves. Our findings show that NTRC is an important mediator of plant responses to microbial VCs through mechanisms involving global thiol redox proteome changes that affect photosynthesis. |
Identificador del proyecto | BIO2013‐ 49125‐C2‐1‐P
BIO2017‐85195‐C2‐1‐P BIO2016‐78747‐P CZ.02.1.01/0.0/0.0/16_019/0000827 LO1204 15H02486 P1004 PROMEBIO P1044 AGROESTI |
Cita | Ameztoy, K., Baslam, M., Sánchez López, Á.M., Muñoz, F.J., Bahaji, A., Almagro, G.,...,Pozueta Romero, J. (2019). Plant responses to fungal volatiles involve global posttranslational thiol redox proteome changes that affect photosynthesis. Plant, Cell and Environment, 42 (9), 2627-2644. |
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