Artículo
Platelet-activating Factor Receptor Initiates Contact of Acinetobacter baumannii Expressing Phosphorylcholine with Host Cells
Autor/es | Smani, Younes
Docobo Pérez, Fernando López Rojas, Rafael Domínguez Herrera, Juan Ibáñez Martínez, José Pachón Díaz, Jerónimo |
Departamento | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica Universidad de Sevilla. Departamento de Medicina |
Fecha de publicación | 2012-06 |
Fecha de depósito | 2021-05-10 |
Publicado en |
|
Resumen | Adhesion is an initial and important step in Acinetobacter baumannii causing infections. However, the exact molecular mechanism of such a step between A. baumannii and the host cells remains unclear. Here, we demonstrated ... Adhesion is an initial and important step in Acinetobacter baumannii causing infections. However, the exact molecular mechanism of such a step between A. baumannii and the host cells remains unclear. Here, we demonstrated that the phosphorylcholine (ChoP)-containing outer membrane protein of A. baumannii binds to A549 cells through platelet-activating factor receptor (PAFR), resulting in activation of G protein and intracellular calcium. Upon A. baumannii expressing ChoP binding to PAFR, clathrin and β-arrestins, proteins involved in the direction of the vacuolar movement, are activated during invasion of A. baumannii. PAFR antagonism restricts the dissemination of A. baumannii in the pneumonia model. These results define a role for PAFR in A. baumannii interaction with host cells and suggest a mechanism for the entry of A. baumannii into the cytoplasm of host cells. |
Cita | Smani, Y., Docobo Pérez, F., López Rojas, R., Domínguez Herrera, J., Ibáñez Martínez, J. y Pachón Díaz, J. (2012). Platelet-activating Factor Receptor Initiates Contact of Acinetobacter baumannii Expressing Phosphorylcholine with Host Cells. Journal Of Biological Chemistry, 287 (32), 26901-26910. |
Ficheros | Tamaño | Formato | Ver | Descripción |
---|---|---|---|---|
Platelet.pdf | 1.865Mb | [PDF] | Ver/ | |